Streptococcal protective antigens (Spa)

A new family of type-specific proteins of group A streptococci

E. A. Ahmed, T. A. Penfound, S. C. Brewer, P. A. Tennant, E. Y. Chiang, James Dale

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Previous studies in our laboratory described a new group A streptococcal protective antigen (Spa) in type 18 streptococci that was distinct from the type 18 M protein. This study was undertaken to identify additional serotypes of group A streptococci that express Spa proteins. PCR techniques were used to identify and clone a new spa gene from type 36 streptococci. The 5′ sequence of spa36 was highly variable compared to spa18, while the 3′ sequence was conserved. Antisera against Spa36 opsonized type 36 streptococci but not type 18 streptococci, indicating that the opsonic Spa epitopes were type-specific. Antisera against the conserved carboxy-terminal half of Spa18 were used to identify Spa or Spa-like proteins expressed on the surface of 25 of 70 different serotypes of GAS. Spa proteins may represent a new family of type-specific surface antigens that function in concert with M proteins to elicit protective immune responses.

Original languageEnglish (US)
Pages (from-to)51-57
Number of pages7
JournalEuropean Journal of Clinical Microbiology and Infectious Diseases
Volume29
Issue number1
DOIs
StatePublished - Jan 1 2010

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Streptococcus
Proteins
Immune Sera
Conserved Sequence
Surface Antigens
streptococcal protective antigen
Epitopes
Clone Cells
Polymerase Chain Reaction
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Streptococcal protective antigens (Spa) : A new family of type-specific proteins of group A streptococci. / Ahmed, E. A.; Penfound, T. A.; Brewer, S. C.; Tennant, P. A.; Chiang, E. Y.; Dale, James.

In: European Journal of Clinical Microbiology and Infectious Diseases, Vol. 29, No. 1, 01.01.2010, p. 51-57.

Research output: Contribution to journalArticle

Ahmed, E. A. ; Penfound, T. A. ; Brewer, S. C. ; Tennant, P. A. ; Chiang, E. Y. ; Dale, James. / Streptococcal protective antigens (Spa) : A new family of type-specific proteins of group A streptococci. In: European Journal of Clinical Microbiology and Infectious Diseases. 2010 ; Vol. 29, No. 1. pp. 51-57.
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