Structural and biomechanical characterizations of porcine myocardial extracellular matrix

Bo Wang, Mary E. Tedder, Clara E. Perez, Guangjun Wang, Amy Curry, Filip To, Steven H. Elder, Lakiesha N. Williams, Dan T. Simionescu, Jun Liao

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Extracellular matrix (ECM) of myocardium plays an important role to maintain a multilayered helical architecture of cardiomyocytes. In this study, we have characterized the structural and biomechanical properties of porcine myocardial ECM. Fresh myocardium were decellularized in a rotating bioreactor using 0.1 % sodium dodecyl sulfate solution. Masson's trichrome staining and SEM demonstrated the removal of cells and preservation of the interconnected 3D cardiomyocyte lacunae. Movat's pentachrome staining showed the preservation of cardiac elastin ultrastructure and vascular elastin distribution/ alignment. DNA assay result confirmed a 98.59 % reduction in DNA content; the acellular myocardial scaffolds were found completely lack of staining for the porcine a-Gal antigen; and the accelerating enzymatic degradation assessment showed a constant degradation rate. Tensile and shear properties of the acellular myocardial scaffolds were also evaluated. Our observations showed that the acellular myocardial ECM possessed important traits of biodegradable scaffolds, indicating the potentials in cardiac regeneration and whole heart tissue engineering.

Original languageEnglish (US)
Pages (from-to)1835-1847
Number of pages13
JournalJournal of Materials Science: Materials in Medicine
Volume23
Issue number8
DOIs
StatePublished - Aug 1 2012
Externally publishedYes

Fingerprint

Elastin
Scaffolds
Extracellular Matrix
DNA
Swine
Staining and Labeling
Cardiac Myocytes
Degradation
Myocardium
Bioelectric potentials
Sodium dodecyl sulfate
Scaffolds (biology)
Antigens
Bioreactors
Tissue engineering
Sodium Dodecyl Sulfate
Assays
Tissue Engineering
Scanning electron microscopy
Blood Vessels

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Structural and biomechanical characterizations of porcine myocardial extracellular matrix. / Wang, Bo; Tedder, Mary E.; Perez, Clara E.; Wang, Guangjun; Curry, Amy; To, Filip; Elder, Steven H.; Williams, Lakiesha N.; Simionescu, Dan T.; Liao, Jun.

In: Journal of Materials Science: Materials in Medicine, Vol. 23, No. 8, 01.08.2012, p. 1835-1847.

Research output: Contribution to journalArticle

Wang, B, Tedder, ME, Perez, CE, Wang, G, Curry, A, To, F, Elder, SH, Williams, LN, Simionescu, DT & Liao, J 2012, 'Structural and biomechanical characterizations of porcine myocardial extracellular matrix', Journal of Materials Science: Materials in Medicine, vol. 23, no. 8, pp. 1835-1847. https://doi.org/10.1007/s10856-012-4660-0
Wang, Bo ; Tedder, Mary E. ; Perez, Clara E. ; Wang, Guangjun ; Curry, Amy ; To, Filip ; Elder, Steven H. ; Williams, Lakiesha N. ; Simionescu, Dan T. ; Liao, Jun. / Structural and biomechanical characterizations of porcine myocardial extracellular matrix. In: Journal of Materials Science: Materials in Medicine. 2012 ; Vol. 23, No. 8. pp. 1835-1847.
@article{874df43009de44fe912f9cae6e937524,
title = "Structural and biomechanical characterizations of porcine myocardial extracellular matrix",
abstract = "Extracellular matrix (ECM) of myocardium plays an important role to maintain a multilayered helical architecture of cardiomyocytes. In this study, we have characterized the structural and biomechanical properties of porcine myocardial ECM. Fresh myocardium were decellularized in a rotating bioreactor using 0.1 {\%} sodium dodecyl sulfate solution. Masson's trichrome staining and SEM demonstrated the removal of cells and preservation of the interconnected 3D cardiomyocyte lacunae. Movat's pentachrome staining showed the preservation of cardiac elastin ultrastructure and vascular elastin distribution/ alignment. DNA assay result confirmed a 98.59 {\%} reduction in DNA content; the acellular myocardial scaffolds were found completely lack of staining for the porcine a-Gal antigen; and the accelerating enzymatic degradation assessment showed a constant degradation rate. Tensile and shear properties of the acellular myocardial scaffolds were also evaluated. Our observations showed that the acellular myocardial ECM possessed important traits of biodegradable scaffolds, indicating the potentials in cardiac regeneration and whole heart tissue engineering.",
author = "Bo Wang and Tedder, {Mary E.} and Perez, {Clara E.} and Guangjun Wang and Amy Curry and Filip To and Elder, {Steven H.} and Williams, {Lakiesha N.} and Simionescu, {Dan T.} and Jun Liao",
year = "2012",
month = "8",
day = "1",
doi = "10.1007/s10856-012-4660-0",
language = "English (US)",
volume = "23",
pages = "1835--1847",
journal = "Journal of Materials Science: Materials in Medicine",
issn = "0957-4530",
publisher = "Springer Netherlands",
number = "8",

}

TY - JOUR

T1 - Structural and biomechanical characterizations of porcine myocardial extracellular matrix

AU - Wang, Bo

AU - Tedder, Mary E.

AU - Perez, Clara E.

AU - Wang, Guangjun

AU - Curry, Amy

AU - To, Filip

AU - Elder, Steven H.

AU - Williams, Lakiesha N.

AU - Simionescu, Dan T.

AU - Liao, Jun

PY - 2012/8/1

Y1 - 2012/8/1

N2 - Extracellular matrix (ECM) of myocardium plays an important role to maintain a multilayered helical architecture of cardiomyocytes. In this study, we have characterized the structural and biomechanical properties of porcine myocardial ECM. Fresh myocardium were decellularized in a rotating bioreactor using 0.1 % sodium dodecyl sulfate solution. Masson's trichrome staining and SEM demonstrated the removal of cells and preservation of the interconnected 3D cardiomyocyte lacunae. Movat's pentachrome staining showed the preservation of cardiac elastin ultrastructure and vascular elastin distribution/ alignment. DNA assay result confirmed a 98.59 % reduction in DNA content; the acellular myocardial scaffolds were found completely lack of staining for the porcine a-Gal antigen; and the accelerating enzymatic degradation assessment showed a constant degradation rate. Tensile and shear properties of the acellular myocardial scaffolds were also evaluated. Our observations showed that the acellular myocardial ECM possessed important traits of biodegradable scaffolds, indicating the potentials in cardiac regeneration and whole heart tissue engineering.

AB - Extracellular matrix (ECM) of myocardium plays an important role to maintain a multilayered helical architecture of cardiomyocytes. In this study, we have characterized the structural and biomechanical properties of porcine myocardial ECM. Fresh myocardium were decellularized in a rotating bioreactor using 0.1 % sodium dodecyl sulfate solution. Masson's trichrome staining and SEM demonstrated the removal of cells and preservation of the interconnected 3D cardiomyocyte lacunae. Movat's pentachrome staining showed the preservation of cardiac elastin ultrastructure and vascular elastin distribution/ alignment. DNA assay result confirmed a 98.59 % reduction in DNA content; the acellular myocardial scaffolds were found completely lack of staining for the porcine a-Gal antigen; and the accelerating enzymatic degradation assessment showed a constant degradation rate. Tensile and shear properties of the acellular myocardial scaffolds were also evaluated. Our observations showed that the acellular myocardial ECM possessed important traits of biodegradable scaffolds, indicating the potentials in cardiac regeneration and whole heart tissue engineering.

UR - http://www.scopus.com/inward/record.url?scp=84864915724&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864915724&partnerID=8YFLogxK

U2 - 10.1007/s10856-012-4660-0

DO - 10.1007/s10856-012-4660-0

M3 - Article

C2 - 22584822

AN - SCOPUS:84864915724

VL - 23

SP - 1835

EP - 1847

JO - Journal of Materials Science: Materials in Medicine

JF - Journal of Materials Science: Materials in Medicine

SN - 0957-4530

IS - 8

ER -