Structural characterization of immunodominant regions of streptokinase recognized by murine monoclonal antibodies

Behnaz Parhami-Seren, Tracy Keel, Guy Reed

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In order to determine the nature of the antigenic and functional determinants of streptokinase (SK), we produced monoclonal antibodies (MAbs) by immunizing A/J mice with native SK protein. By virtue of their differential binding to a large panel of recombinant SK truncated proteins, and their effect on the formation of functional SK-plasminogen activator complex (SKPAC), these MAbs were found to recognize 6 unique and minimally overlapping epitopes on the SK protein. The fine epitope specificity of the anti-SK MAbs derived from A/J mice was compared with that of MAbs derived from BALB/c mice. A number of MAbs from both inbred strains of mice were directed against the same sequences of SK (1-13, 1-253, 120-352) suggesting that these regions of the molecule contain peptide sequences that are immunodominant. Two of the 'immunodominant' sequences of SK protein appeared to be important for SK function, since the formation of SKPAC could be inhibited by MAbs against these sequences.

Original languageEnglish (US)
Pages (from-to)169-176
Number of pages8
JournalHybridoma
Volume15
Issue number3
DOIs
StatePublished - Jan 1 1996

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Immunodominant Epitopes
Streptokinase
Monoclonal Antibodies
Epitopes
Proteins
Inbred Strains Mice
Plasminogen Activators
Peptides

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Structural characterization of immunodominant regions of streptokinase recognized by murine monoclonal antibodies. / Parhami-Seren, Behnaz; Keel, Tracy; Reed, Guy.

In: Hybridoma, Vol. 15, No. 3, 01.01.1996, p. 169-176.

Research output: Contribution to journalArticle

Parhami-Seren, Behnaz ; Keel, Tracy ; Reed, Guy. / Structural characterization of immunodominant regions of streptokinase recognized by murine monoclonal antibodies. In: Hybridoma. 1996 ; Vol. 15, No. 3. pp. 169-176.
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