Structural studies of an Inv (1, -2) kappa light chain

Arhur G. Steinberg, Celia P. Milstein, Carla L. McLaughlin, Alan Solomon

Research output: Contribution to journalArticle

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Abstract

A κ Bence Jones protein with phenotype Inv (1, -2) was isolated from the urine of a patient with multiple myeloma. Inv typing of the patient's relatives established the presence of an Inv1 allele in the kindred, and that the patient's genotype was Inv1/Inv3. Hence, the absence of Inv (2) in the Bence Jones protein was shown to be genetic and not an artifact caused by the disease. The tryptic peptide-containing residues 191 through 194 were isolated and shown to be composed of Leu, Tyr, Ala, Cys, with Leu at the amino end. Hence, the residue at 191 is the same as that present in Inv (1, 2) Bence Jones proteins. More detailed study of the tryptic peptides established that residue 153 is Val rather than Ala as in all other K chains thus far studied. The primary sequence: Ala153, Leu191 determines Inv (1, 2); Ala153, Val191 determines Inv (3); and Val153, Leu191 determines Inv (1). The Val153, Val191 sequence has not been observed. It may correspond to Inv (-). These data are strikingly similar to the data for the Kern and Oz isotypes (changes at 154 and 191, respectively) in the λ chain. As in the case of the K chain, only three of the four possible combinations have been observed. The implications of this parallelism and of crystallographic findings on λ chains, reported by others, are discussed.

Original languageEnglish (US)
Pages (from-to)108-117
Number of pages10
JournalImmunogenetics
Volume1
Issue number1
DOIs
StatePublished - Dec 1 1974

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Bence Jones Protein
Light
Peptides
Multiple Myeloma
Artifacts
Alleles
Genotype
Urine
Phenotype

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Steinberg, A. G., Milstein, C. P., McLaughlin, C. L., & Solomon, A. (1974). Structural studies of an Inv (1, -2) kappa light chain. Immunogenetics, 1(1), 108-117. https://doi.org/10.1007/BF01564051

Structural studies of an Inv (1, -2) kappa light chain. / Steinberg, Arhur G.; Milstein, Celia P.; McLaughlin, Carla L.; Solomon, Alan.

In: Immunogenetics, Vol. 1, No. 1, 01.12.1974, p. 108-117.

Research output: Contribution to journalArticle

Steinberg, AG, Milstein, CP, McLaughlin, CL & Solomon, A 1974, 'Structural studies of an Inv (1, -2) kappa light chain', Immunogenetics, vol. 1, no. 1, pp. 108-117. https://doi.org/10.1007/BF01564051
Steinberg, Arhur G. ; Milstein, Celia P. ; McLaughlin, Carla L. ; Solomon, Alan. / Structural studies of an Inv (1, -2) kappa light chain. In: Immunogenetics. 1974 ; Vol. 1, No. 1. pp. 108-117.
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