Structural studies of Hantaan virus

Anthony J. Battisti, Yong Kyu Chu, Paul R. Chipman, Bärbel Kaufmann, Colleen Jonsson, Michael G. Rossmann

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Hantaan virus is the prototypic member of the Hantavirus genus within the family Bunyaviridae and is a causative agent of the potentially fatal hemorrhagic fever with renal syndrome. The Bunyaviridae are a family of negative-sense RNA viruses with three-part segmented genomes. Virions are enveloped and decorated with spikes derived from a pair of glycoproteins (Gn and Gc). Here, we present cryo-electron tomography and single-particle cryo-electron microscopy studies of Hantaan virus virions. We have determined the structure of the tetrameric Gn-Gc spike complex to a resolution of 2.5 nm and show that spikes are ordered in lattices on the virion surface. Large cytoplasmic extensions associated with each Gn-Gc spike also form a lattice on the inner surface of the viral membrane. Rod-shaped ribonucleoprotein complexes are arranged into nearly parallel pairs and triplets within virions. Our results differ from the T=12 icosahedral organization found for some bunyaviruses. However, a comparison of our results with the previous tomographic studies of the nonpathogenic Tula hantavirus indicates a common structural organization for hantaviruses.

Original languageEnglish (US)
Pages (from-to)835-841
Number of pages7
JournalJournal of Virology
Volume85
Issue number2
DOIs
StatePublished - Jan 1 2011

Fingerprint

Hantaan virus
Hantavirus
virion
Virion
Bunyaviridae
Orthobunyavirus
Electron Microscope Tomography
Hemorrhagic Fever with Renal Syndrome
Cryoelectron Microscopy
ribonucleoproteins
Ribonucleoproteins
RNA Viruses
tomography
fever
glycoproteins
Glycoproteins
kidneys
electrons
Genome
genome

All Science Journal Classification (ASJC) codes

  • Immunology
  • Virology

Cite this

Battisti, A. J., Chu, Y. K., Chipman, P. R., Kaufmann, B., Jonsson, C., & Rossmann, M. G. (2011). Structural studies of Hantaan virus. Journal of Virology, 85(2), 835-841. https://doi.org/10.1128/JVI.01847-10

Structural studies of Hantaan virus. / Battisti, Anthony J.; Chu, Yong Kyu; Chipman, Paul R.; Kaufmann, Bärbel; Jonsson, Colleen; Rossmann, Michael G.

In: Journal of Virology, Vol. 85, No. 2, 01.01.2011, p. 835-841.

Research output: Contribution to journalArticle

Battisti, AJ, Chu, YK, Chipman, PR, Kaufmann, B, Jonsson, C & Rossmann, MG 2011, 'Structural studies of Hantaan virus', Journal of Virology, vol. 85, no. 2, pp. 835-841. https://doi.org/10.1128/JVI.01847-10
Battisti AJ, Chu YK, Chipman PR, Kaufmann B, Jonsson C, Rossmann MG. Structural studies of Hantaan virus. Journal of Virology. 2011 Jan 1;85(2):835-841. https://doi.org/10.1128/JVI.01847-10
Battisti, Anthony J. ; Chu, Yong Kyu ; Chipman, Paul R. ; Kaufmann, Bärbel ; Jonsson, Colleen ; Rossmann, Michael G. / Structural studies of Hantaan virus. In: Journal of Virology. 2011 ; Vol. 85, No. 2. pp. 835-841.
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