Structural vs. contractile protein remodeling and myocardial stiffness in hypertrophied rat left ventricle

Jorge E. Jalil, Christian W. Doering, Joseph S. Janicki, Ruth Pick, William A. Clark, Cyril Abrahams, Karl Weber

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Left ventricular pressure overload will result in the hypertrophic growth of the myocardium and in the rat may include a remodeling of both the structural and contractile proteins. As a result, an adaptive rise in active stiffness, or the force generating capacity of the myocardium, may occur. The relative importance of structural vs. contractile protein remodeling to the hypertrophic response and active stiffness is unclear. Accordingly, we monitored the ratio of V1 V3 isomyosin and the fibrillar nature and volume fraction of myocardial collagen, together with the developed systolic stress-strain relation of the intact myocardium in the adult male Wistar rat after 8 weeks of abdominal aorta banding. In comparison to controls and for the 20% increase in left ventricular mass obtained with banding we found: (a) collagen volume fraction had increased significantly (6.2 ± 2.0 vs. 3.6 ± 1.0%) while the V1 V3 ratio did not change; (b) interstitial compartment remodeling included a perivascular accumulation of collagen around small intramyocardial coronary arteries and the appearance of more extensive fibrillar collagens; and (c) active stiffness increased significantly. Thus, the increase in active stiffness of the hypertrophied adult rat myocardium, seen with abdominal aorta banding, appears to be related to interstitial fibrosis and not a conversion of myosin isoforms.

Original languageEnglish (US)
Pages (from-to)1179-1187
Number of pages9
JournalJournal of Molecular and Cellular Cardiology
Volume20
Issue number12
DOIs
StatePublished - Jan 1 1988

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Contractile Proteins
Heart Ventricles
Myocardium
Collagen
Abdominal Aorta
Fibrillar Collagens
Ventricular Pressure
Myosins
Wistar Rats
Coronary Vessels
Protein Isoforms
Fibrosis
Growth

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Structural vs. contractile protein remodeling and myocardial stiffness in hypertrophied rat left ventricle. / Jalil, Jorge E.; Doering, Christian W.; Janicki, Joseph S.; Pick, Ruth; Clark, William A.; Abrahams, Cyril; Weber, Karl.

In: Journal of Molecular and Cellular Cardiology, Vol. 20, No. 12, 01.01.1988, p. 1179-1187.

Research output: Contribution to journalArticle

Jalil, Jorge E. ; Doering, Christian W. ; Janicki, Joseph S. ; Pick, Ruth ; Clark, William A. ; Abrahams, Cyril ; Weber, Karl. / Structural vs. contractile protein remodeling and myocardial stiffness in hypertrophied rat left ventricle. In: Journal of Molecular and Cellular Cardiology. 1988 ; Vol. 20, No. 12. pp. 1179-1187.
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