Structure, activity, and distribution of fish osteocalcin

Satoru Nishimoto, J. Herbert Waite, Miyako Nishimoto, Richard W. Kriwacki

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Osteocalcin (bone Gla protein) is an extracellular matrix protein synthesized by osteoblasts that is a marker of bone. Osteocalcin probably originated in the ancestors of Teleostei or bony fish and of the Tetrapoda or amphibians, reptiles, birds, and mammals. We have characterized the Cyprinus carpio (carp) osteocalcin for mineral binding to hydroxyapatite, amino acid sequence, and extent of secondary structure. Hydroxyapatite binding is enhanced in the presence of calcium. The α-helical content of teleost osteocalcin increases and β-sheet structure decreases upon calcium binding, similar to findings in calf osteocalcin. The gene structure and primary sequence of prepro-osteocalcin from 2 pufferfish compared with carp shows that there are many conserved features in teleost osteocalcin genes. Using an immunoassay for carp osteocalcin, we determined that the relative content of osteocalcin is highest in dorsal fin spines and other bones and lowest in scales. The carp osteocalcin antibodies, cross-reactive to other species of fish, were used to study the role of osteocalcin in teleost model systems.

Original languageEnglish (US)
Pages (from-to)11843-11848
Number of pages6
JournalJournal of Biological Chemistry
Volume278
Issue number14
DOIs
StatePublished - Apr 4 2003

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Osteocalcin
Fish
Fishes
Durapatite
Bone
Genes
Tetraodontiformes
Calcium
Bone and Bones
Mammals
Reptiles
Carps
Extracellular Matrix Proteins
Osteoblasts
Birds
Amphibians
Immunoassay
Minerals
Amino Acid Sequence
Spine

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Structure, activity, and distribution of fish osteocalcin. / Nishimoto, Satoru; Waite, J. Herbert; Nishimoto, Miyako; Kriwacki, Richard W.

In: Journal of Biological Chemistry, Vol. 278, No. 14, 04.04.2003, p. 11843-11848.

Research output: Contribution to journalArticle

Nishimoto, Satoru ; Waite, J. Herbert ; Nishimoto, Miyako ; Kriwacki, Richard W. / Structure, activity, and distribution of fish osteocalcin. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 14. pp. 11843-11848.
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