Structure and biochemical properties of the alkene producing cytochrome p450 OleTJE (CYP152l1) from the jeotgalicoccus sp. 8456 bacterium

James Belcher, Kirsty J. McLean, Sarah Matthews, Laura S. Woodward, Karl Fisher, Stephen E.J. Rigby, David Nelson, Donna Potts, Michael T. Baynham, David A. Parker, David Leys, Andrew W. Munro

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Background: OleTJE oxidatively decarboxylates fatty acids to produce terminal alkenes. Results: OleTJE is an efficient peroxide-dependent lipid decarboxylase, with high affinity substrate binding and the capacity to be resolubilized from precipitate in an active form. Conclusion: OleTJE has key differences in active site structure and substrate binding/mechanistic properties from related CYP152 hydroxylases. Significance: OleTJE is an efficient and robust biocatalyst with applications in biofuel production.

Original languageEnglish (US)
Pages (from-to)6535-6550
Number of pages16
JournalJournal of Biological Chemistry
Volume289
Issue number10
DOIs
StatePublished - Mar 7 2014

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Carboxy-Lyases
Biofuels
Lipid Peroxides
Alkenes
Mixed Function Oxygenases
Cytochrome P-450 Enzyme System
Catalytic Domain
Bacteria
Fatty Acids
Peroxides
Substrates
Enzymes
Precipitates
Lipids

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Structure and biochemical properties of the alkene producing cytochrome p450 OleTJE (CYP152l1) from the jeotgalicoccus sp. 8456 bacterium. / Belcher, James; McLean, Kirsty J.; Matthews, Sarah; Woodward, Laura S.; Fisher, Karl; Rigby, Stephen E.J.; Nelson, David; Potts, Donna; Baynham, Michael T.; Parker, David A.; Leys, David; Munro, Andrew W.

In: Journal of Biological Chemistry, Vol. 289, No. 10, 07.03.2014, p. 6535-6550.

Research output: Contribution to journalArticle

Belcher, J, McLean, KJ, Matthews, S, Woodward, LS, Fisher, K, Rigby, SEJ, Nelson, D, Potts, D, Baynham, MT, Parker, DA, Leys, D & Munro, AW 2014, 'Structure and biochemical properties of the alkene producing cytochrome p450 OleTJE (CYP152l1) from the jeotgalicoccus sp. 8456 bacterium', Journal of Biological Chemistry, vol. 289, no. 10, pp. 6535-6550. https://doi.org/10.1074/jbc.M113.527325
Belcher, James ; McLean, Kirsty J. ; Matthews, Sarah ; Woodward, Laura S. ; Fisher, Karl ; Rigby, Stephen E.J. ; Nelson, David ; Potts, Donna ; Baynham, Michael T. ; Parker, David A. ; Leys, David ; Munro, Andrew W. / Structure and biochemical properties of the alkene producing cytochrome p450 OleTJE (CYP152l1) from the jeotgalicoccus sp. 8456 bacterium. In: Journal of Biological Chemistry. 2014 ; Vol. 289, No. 10. pp. 6535-6550.
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