Structure of clonal and polyclonal cell arrays in chimeric mouse retina

Robert Williams, Dan Goldowitz

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

One of the most striking results of recent cell-lineage studies of vertebrate retina is the marked variability in the size and types of clones marked by retroviral transfection and dye injection of embryonic progenitor cells. Is this variability due to microenvironmental modulation of cell determination, to lineage restriction, or to experimental perturbation of the progenitor cells? We have taken advantage of species-specific DNA probes to mark groups of lineage-related cells in experimental mouse chimeras. This method of marking cells has two distinct advantages over previous methods: direct manipulation of progenitor cells is avoided, and clones are established at an earlier stage of retinal development. The most notable feature of retinal cohorts in chimeras is their structural uniformity - each is a solid radial array that contains the same ratio of major cell types as the retina itself. This is true even of the smallest monoclonal cohorts, which contain fewer than 200 cells. Our result provides compelling empirical support for the hypothesis that the murine retina is made up of hundreds of relatively homogeneous radial units, each derived from single retinal precursor cells. This finding is inconsistent with microenvironmental modulation of clone structure early in development. We raise the possibility that the heterogeneity among clones marked by dye injection and transfection is due to progressive lineage restriction or to experimental perturbation of the retinal progenitor cells.

Original languageEnglish (US)
Pages (from-to)1184-1188
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number4
DOIs
StatePublished - Feb 15 1992

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Retina
Stem Cells
Clone Cells
Transfection
Coloring Agents
Injections
DNA Probes
Cell Lineage
Vertebrates

All Science Journal Classification (ASJC) codes

  • General

Cite this

Structure of clonal and polyclonal cell arrays in chimeric mouse retina. / Williams, Robert; Goldowitz, Dan.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 4, 15.02.1992, p. 1184-1188.

Research output: Contribution to journalArticle

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