Studies of naloxone-induced secretion of β-endorphin immunoreactivity in dogs

Ellis R. Levin, Burt Sharp, Harold E. Carlson

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The stimulating effect of naloxone on plasma β-endorphin immunoreactivity (βEI) was examined in dogs. Intravenous naloxone at 5, 1, or 0.1 mg/kg caused a significant increase in βEI while doses of 0.01 or 0.001 mg/kg had no effect. The peak plasma βEI levels occurred at 25 mins after naloxone. The neurotransmitter and antagonists metergoline, atropine, diphenhydramine and phentolamine all failed to significantly alter basal βEI secretion; further, they all failed to prevent the increase in βEI resulting from naloxone administration. Dexamethasone prevented the naloxone-induced rise in βEI. Our results suggest naloxone's effect on βEI is not mediated through several neurotransmitter systems known to affect ACTH secretion. Additionally, βEI secreted in response to naloxone appears to originate mainly from the anterior lobe of the pituitary.

Original languageEnglish (US)
Pages (from-to)1535-1545
Number of pages11
JournalLife Sciences
Volume35
Issue number15
DOIs
StatePublished - Oct 8 1984

Fingerprint

Endorphins
Naloxone
Dogs
Neurotransmitter Agents
Metergoline
Plasmas
Diphenhydramine
Anterior Pituitary Gland
Phentolamine
Atropine
Adrenocorticotropic Hormone
Dexamethasone

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Studies of naloxone-induced secretion of β-endorphin immunoreactivity in dogs. / Levin, Ellis R.; Sharp, Burt; Carlson, Harold E.

In: Life Sciences, Vol. 35, No. 15, 08.10.1984, p. 1535-1545.

Research output: Contribution to journalArticle

Levin, Ellis R. ; Sharp, Burt ; Carlson, Harold E. / Studies of naloxone-induced secretion of β-endorphin immunoreactivity in dogs. In: Life Sciences. 1984 ; Vol. 35, No. 15. pp. 1535-1545.
@article{5989a3238b6144739d66aa4e2098e38e,
title = "Studies of naloxone-induced secretion of β-endorphin immunoreactivity in dogs",
abstract = "The stimulating effect of naloxone on plasma β-endorphin immunoreactivity (βEI) was examined in dogs. Intravenous naloxone at 5, 1, or 0.1 mg/kg caused a significant increase in βEI while doses of 0.01 or 0.001 mg/kg had no effect. The peak plasma βEI levels occurred at 25 mins after naloxone. The neurotransmitter and antagonists metergoline, atropine, diphenhydramine and phentolamine all failed to significantly alter basal βEI secretion; further, they all failed to prevent the increase in βEI resulting from naloxone administration. Dexamethasone prevented the naloxone-induced rise in βEI. Our results suggest naloxone's effect on βEI is not mediated through several neurotransmitter systems known to affect ACTH secretion. Additionally, βEI secreted in response to naloxone appears to originate mainly from the anterior lobe of the pituitary.",
author = "Levin, {Ellis R.} and Burt Sharp and Carlson, {Harold E.}",
year = "1984",
month = "10",
day = "8",
doi = "10.1016/0024-3205(84)90351-5",
language = "English (US)",
volume = "35",
pages = "1535--1545",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "15",

}

TY - JOUR

T1 - Studies of naloxone-induced secretion of β-endorphin immunoreactivity in dogs

AU - Levin, Ellis R.

AU - Sharp, Burt

AU - Carlson, Harold E.

PY - 1984/10/8

Y1 - 1984/10/8

N2 - The stimulating effect of naloxone on plasma β-endorphin immunoreactivity (βEI) was examined in dogs. Intravenous naloxone at 5, 1, or 0.1 mg/kg caused a significant increase in βEI while doses of 0.01 or 0.001 mg/kg had no effect. The peak plasma βEI levels occurred at 25 mins after naloxone. The neurotransmitter and antagonists metergoline, atropine, diphenhydramine and phentolamine all failed to significantly alter basal βEI secretion; further, they all failed to prevent the increase in βEI resulting from naloxone administration. Dexamethasone prevented the naloxone-induced rise in βEI. Our results suggest naloxone's effect on βEI is not mediated through several neurotransmitter systems known to affect ACTH secretion. Additionally, βEI secreted in response to naloxone appears to originate mainly from the anterior lobe of the pituitary.

AB - The stimulating effect of naloxone on plasma β-endorphin immunoreactivity (βEI) was examined in dogs. Intravenous naloxone at 5, 1, or 0.1 mg/kg caused a significant increase in βEI while doses of 0.01 or 0.001 mg/kg had no effect. The peak plasma βEI levels occurred at 25 mins after naloxone. The neurotransmitter and antagonists metergoline, atropine, diphenhydramine and phentolamine all failed to significantly alter basal βEI secretion; further, they all failed to prevent the increase in βEI resulting from naloxone administration. Dexamethasone prevented the naloxone-induced rise in βEI. Our results suggest naloxone's effect on βEI is not mediated through several neurotransmitter systems known to affect ACTH secretion. Additionally, βEI secreted in response to naloxone appears to originate mainly from the anterior lobe of the pituitary.

UR - http://www.scopus.com/inward/record.url?scp=0021132771&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021132771&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(84)90351-5

DO - 10.1016/0024-3205(84)90351-5

M3 - Article

VL - 35

SP - 1535

EP - 1545

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 15

ER -