Studies on a nuclear polyhedrosis virus in Bombyx mori cells in vitro. 1. Multiplication kinetics and ultrastructural studies

Rajendra Raghow, T. D.C. Grace

Research output: Contribution to journalArticle

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Abstract

The analysis of the multiplication kinetics of Bombyx mori nuclear polyhedrosis virus (BMNPV) in Grace's B. mori cell line reveals three phases of viral development: (a) a latent phase (0-12 hours), (b) an exponential phase (16-48 hours), and (c) a stationary phase (48 hours onwards). Ultrastructural observations of infected cells at various times during these three phases reveal a sequence of events of virus replication as follows: (i) Membrane-enclosed virus particles enter the cell by phagocytosis and viropexis (in the early part of the latent phase). Naked NPV rods are seen attached to the nuclear membrane in the nuclear pore area. (ii) The precursor of the virogenic stroma (PVS) appears as electron-dense patches in the nucleus 16 hours after infection (beginning of the exponential phase). It changes into an electron-dense network associated with large numbers of naked viral rods 32 hours after infection. (iii) About 90% of the virions become membrane-enclosed (36 hours after infection) followed by their occlusion into polyhedra (40 hours after infection). (iv) The maximum titre is attained 48 hours after infection. Paracrystalline arrays of tubular aberrant forms, probably of viral origin, and proliferation of membrane profiles were observed in the infected cells.

Original languageEnglish (US)
Pages (from-to)384-399
Number of pages16
JournalJournal of Ultrasructure Research
Volume47
Issue number3
DOIs
StatePublished - Jan 1 1974

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Nucleopolyhedrovirus
Bombyx
Infection
Virion
Membranes
Electrons
Nuclear Pore
Cytophagocytosis
Nuclear Envelope
Virus Replication
In Vitro Techniques
Cell Line

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Molecular Biology

Cite this

Studies on a nuclear polyhedrosis virus in Bombyx mori cells in vitro. 1. Multiplication kinetics and ultrastructural studies. / Raghow, Rajendra; Grace, T. D.C.

In: Journal of Ultrasructure Research, Vol. 47, No. 3, 01.01.1974, p. 384-399.

Research output: Contribution to journalArticle

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