Studies on glycosaminoglycans of regenerating rabbit ear cartilage

Karen Hasty, Gerald N. Smith, Andrew Kang

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Cartilage regeneration in the adult rabbit ear was examined with respect to glycosaminoglycan (GAG) synthesis at various stages of the regeneration process. Increased hyaluronic acid and chondroitin sulfate synthesis was first seen 31 days after wounding, when a metachromatic cartilage matrix could be distinguished from blastemal cells. Analysis of cartilage and the overlying skin separately showed that 90% of the labeled chondroitin sulfate was found in the cartilage being regenerated. DEAE-cellulose chromatography of GAG preparations from 35-day regenerating cartilages showed hyaluronic acid and chondroitin sulfate peaks eluting in the same position as those isolated from normal cartilages. The identity of the hyaluronic acid and chondroitin sulfate peaks was confirmed by their susceptibility to Streptomyces hyaluronidase and chondroitinase ABC, respectively. Although the degree of sulfation in normal and regenerated cartilages was similar, the ratio of chondroitin 6-sulfate to chondroitin 4-sulfate was increased in regenerated cartilages. GAG preparations from unlabeled cartilages were digested with chondroitinase ABC and the disaccharide digestive products were identified and quantitiated. Normal cartilage had a ΔDi-6S ΔDi-4S ratio of 0.27; the same ratio for the regenerated cartilage was 1.58.

Original languageEnglish (US)
Pages (from-to)198-205
Number of pages8
JournalDevelopmental Biology
Volume86
Issue number1
DOIs
StatePublished - Jan 1 1981

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Ear Cartilage
Glycosaminoglycans
Cartilage
Rabbits
Chondroitin Sulfates
Hyaluronic Acid
Chondroitin ABC Lyase
Regeneration
DEAE-Cellulose Chromatography
Hyaluronoglucosaminidase
Disaccharides
Streptomyces
Ear

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Studies on glycosaminoglycans of regenerating rabbit ear cartilage. / Hasty, Karen; Smith, Gerald N.; Kang, Andrew.

In: Developmental Biology, Vol. 86, No. 1, 01.01.1981, p. 198-205.

Research output: Contribution to journalArticle

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