Study of mRNA growth factors in urinary cells of kidney transplant recipients as predictors of chronic allograft nephropathy

Valeria R. Mas, Daniel G. Maluf, Kellie J. Archer, Kenneth Yanek, Anne King, Adrian Cotterell, Andrea Ferreira-Gonzalez, Cheryl Rodgers, Robert A. Fisher, Marc Posner

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background. Chronic allograft nephropathy (CAN) is responsible for a significant proportion of graft loss. Current diagnostic methods for CAN are inadequate, and noninvasive assays for detecting allograft dysfunction/rejection and predicting long-term outcomes are a priority in transplantation. Methods. Urine samples were collected from 48 kidney transplant recipients (KTR): 18 recipients with stable graft function (creatinine levels <2.0 mg/dL) and proteinuria of less than 500 mg/24 hr (Group 1); 18 recipients with stable graft function and proteinuria of greater than 500 mg/24 hr (Group 2); and 12 recipients with biopsy confirmed CAN. Urinary cell levels of transforming growth factor-β1 (TGF-β1) mRNA or epidermal growth factor (EGF) mRNA were measured using real-time quantitative PCR assay, and levels were correlated with renal allograft status. The integrity of RNA isolated from urine sediments was also assessed using the Agilent 2100 Bioanalyzer. Results. Urinary cell TGF-β1 mRNA levels were higher in the CAN group compared to Group 1 (P<0.0001) or Group 2 (P<0.0001). Urinary cell EGF mRNA levels were higher in Group 1 compared to Group 2 (P<0.0001) or the CAN group (P<0.0001). There were no significant differences in the urinary cell levels of EGF mRNA between patients with greater than 500 mg/24 hr proteinuria (Group 2) and the CAN group (P=0.75). Conclusion. These results demonstrate that urinary cell TGF-β1 mRNA levels distinguish CAN patients from long-term transplant patients with stable renal function and varied levels of proteinuria. Urinary cells may be a good resource for the noninvasive diagnosis of CAN.

Original languageEnglish (US)
Pages (from-to)1686-1691
Number of pages6
JournalTransplantation
Volume80
Issue number12
DOIs
StatePublished - Dec 1 2005

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Allografts
Intercellular Signaling Peptides and Proteins
Transplants
Kidney
Messenger RNA
Proteinuria
Transforming Growth Factors
Epidermal Growth Factor
Transplant Recipients
Urine
Real-Time Polymerase Chain Reaction
Creatinine
Transplantation
RNA
Biopsy

All Science Journal Classification (ASJC) codes

  • Transplantation

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Study of mRNA growth factors in urinary cells of kidney transplant recipients as predictors of chronic allograft nephropathy. / Mas, Valeria R.; Maluf, Daniel G.; Archer, Kellie J.; Yanek, Kenneth; King, Anne; Cotterell, Adrian; Ferreira-Gonzalez, Andrea; Rodgers, Cheryl; Fisher, Robert A.; Posner, Marc.

In: Transplantation, Vol. 80, No. 12, 01.12.2005, p. 1686-1691.

Research output: Contribution to journalArticle

Mas, VR, Maluf, DG, Archer, KJ, Yanek, K, King, A, Cotterell, A, Ferreira-Gonzalez, A, Rodgers, C, Fisher, RA & Posner, M 2005, 'Study of mRNA growth factors in urinary cells of kidney transplant recipients as predictors of chronic allograft nephropathy', Transplantation, vol. 80, no. 12, pp. 1686-1691. https://doi.org/10.1097/01.tp.0000185472.79948.db
Mas, Valeria R. ; Maluf, Daniel G. ; Archer, Kellie J. ; Yanek, Kenneth ; King, Anne ; Cotterell, Adrian ; Ferreira-Gonzalez, Andrea ; Rodgers, Cheryl ; Fisher, Robert A. ; Posner, Marc. / Study of mRNA growth factors in urinary cells of kidney transplant recipients as predictors of chronic allograft nephropathy. In: Transplantation. 2005 ; Vol. 80, No. 12. pp. 1686-1691.
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abstract = "Background. Chronic allograft nephropathy (CAN) is responsible for a significant proportion of graft loss. Current diagnostic methods for CAN are inadequate, and noninvasive assays for detecting allograft dysfunction/rejection and predicting long-term outcomes are a priority in transplantation. Methods. Urine samples were collected from 48 kidney transplant recipients (KTR): 18 recipients with stable graft function (creatinine levels <2.0 mg/dL) and proteinuria of less than 500 mg/24 hr (Group 1); 18 recipients with stable graft function and proteinuria of greater than 500 mg/24 hr (Group 2); and 12 recipients with biopsy confirmed CAN. Urinary cell levels of transforming growth factor-β1 (TGF-β1) mRNA or epidermal growth factor (EGF) mRNA were measured using real-time quantitative PCR assay, and levels were correlated with renal allograft status. The integrity of RNA isolated from urine sediments was also assessed using the Agilent 2100 Bioanalyzer. Results. Urinary cell TGF-β1 mRNA levels were higher in the CAN group compared to Group 1 (P<0.0001) or Group 2 (P<0.0001). Urinary cell EGF mRNA levels were higher in Group 1 compared to Group 2 (P<0.0001) or the CAN group (P<0.0001). There were no significant differences in the urinary cell levels of EGF mRNA between patients with greater than 500 mg/24 hr proteinuria (Group 2) and the CAN group (P=0.75). Conclusion. These results demonstrate that urinary cell TGF-β1 mRNA levels distinguish CAN patients from long-term transplant patients with stable renal function and varied levels of proteinuria. Urinary cells may be a good resource for the noninvasive diagnosis of CAN.",
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AU - Mas, Valeria R.

AU - Maluf, Daniel G.

AU - Archer, Kellie J.

AU - Yanek, Kenneth

AU - King, Anne

AU - Cotterell, Adrian

AU - Ferreira-Gonzalez, Andrea

AU - Rodgers, Cheryl

AU - Fisher, Robert A.

AU - Posner, Marc

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Background. Chronic allograft nephropathy (CAN) is responsible for a significant proportion of graft loss. Current diagnostic methods for CAN are inadequate, and noninvasive assays for detecting allograft dysfunction/rejection and predicting long-term outcomes are a priority in transplantation. Methods. Urine samples were collected from 48 kidney transplant recipients (KTR): 18 recipients with stable graft function (creatinine levels <2.0 mg/dL) and proteinuria of less than 500 mg/24 hr (Group 1); 18 recipients with stable graft function and proteinuria of greater than 500 mg/24 hr (Group 2); and 12 recipients with biopsy confirmed CAN. Urinary cell levels of transforming growth factor-β1 (TGF-β1) mRNA or epidermal growth factor (EGF) mRNA were measured using real-time quantitative PCR assay, and levels were correlated with renal allograft status. The integrity of RNA isolated from urine sediments was also assessed using the Agilent 2100 Bioanalyzer. Results. Urinary cell TGF-β1 mRNA levels were higher in the CAN group compared to Group 1 (P<0.0001) or Group 2 (P<0.0001). Urinary cell EGF mRNA levels were higher in Group 1 compared to Group 2 (P<0.0001) or the CAN group (P<0.0001). There were no significant differences in the urinary cell levels of EGF mRNA between patients with greater than 500 mg/24 hr proteinuria (Group 2) and the CAN group (P=0.75). Conclusion. These results demonstrate that urinary cell TGF-β1 mRNA levels distinguish CAN patients from long-term transplant patients with stable renal function and varied levels of proteinuria. Urinary cells may be a good resource for the noninvasive diagnosis of CAN.

AB - Background. Chronic allograft nephropathy (CAN) is responsible for a significant proportion of graft loss. Current diagnostic methods for CAN are inadequate, and noninvasive assays for detecting allograft dysfunction/rejection and predicting long-term outcomes are a priority in transplantation. Methods. Urine samples were collected from 48 kidney transplant recipients (KTR): 18 recipients with stable graft function (creatinine levels <2.0 mg/dL) and proteinuria of less than 500 mg/24 hr (Group 1); 18 recipients with stable graft function and proteinuria of greater than 500 mg/24 hr (Group 2); and 12 recipients with biopsy confirmed CAN. Urinary cell levels of transforming growth factor-β1 (TGF-β1) mRNA or epidermal growth factor (EGF) mRNA were measured using real-time quantitative PCR assay, and levels were correlated with renal allograft status. The integrity of RNA isolated from urine sediments was also assessed using the Agilent 2100 Bioanalyzer. Results. Urinary cell TGF-β1 mRNA levels were higher in the CAN group compared to Group 1 (P<0.0001) or Group 2 (P<0.0001). Urinary cell EGF mRNA levels were higher in Group 1 compared to Group 2 (P<0.0001) or the CAN group (P<0.0001). There were no significant differences in the urinary cell levels of EGF mRNA between patients with greater than 500 mg/24 hr proteinuria (Group 2) and the CAN group (P=0.75). Conclusion. These results demonstrate that urinary cell TGF-β1 mRNA levels distinguish CAN patients from long-term transplant patients with stable renal function and varied levels of proteinuria. Urinary cells may be a good resource for the noninvasive diagnosis of CAN.

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