Study on the effects of heat and ultraviolet light radiation on hepatitis C virus JFH-1 strain inactivation

Hong Shuo Song, Shuang Shi, Ling Yan, Kui Li, Hui Zhuang

Research output: Contribution to journalArticle

Abstract

Objective: To investigate the inactivating effect of heat and ultraviolet (UV) light on HCV JFH-1 strain using the cell culture system. Methods: The HCV JFH-1 virus stock, with an initial titer of 2.5 × 10 4 FFU/ml, was exposed in 56°C water bath or to UV light for varying durations of time for exploring their inactivating effects on the virus. The kinetics of virus titer reduction was determined by an indirect immuno-fluorescence assay (IFA). If the cells infected with the exposed virus stock were IFA negative after three blind passages, the virus stock was considered to be inactivated completely. Results: After incubation of the HCV JFH-1 virus stock (2.5 × 10 4 FFU/ml) in 56°C water bath for 10 min, 20 min and 30 min, the virus titers were reduced to 1.6 × 10 3 FFU/ml, 3.1 × 10 2 FFU/ml and 3.3 × 10 FFU/ml, respectively. The exposure of the virus stock to UV light (wavelength 253.7 nm, intensity ≥60 μW/cm 2, 30 cm below the UV lamp) for 15 s, 30 s and 45 s resulted in virus titer reduction to 1.0 × 10 3 FFU/ml, 1.1 × 10 2 FFU/ml and 2.7 × 10 FFU/ml, respectively. After 40 min incubation of the virus stock at 56°C, or 1 min exposure to UV light (wavelength 253.7 nm, intensity ≥60 μW/cm 2) the virus infectious titer was reduced below the detection limit of IFA, and the IFA was still negative even after three blind passages, indicating that the virus was inactivated completely. Conclusion: HCV is sensitive to heat and UV light treatment. For HCV JFH-1 virus stock containing 2.5 × 10 4 FFU/ml virus, heat treatment at 56°C for 40 min, or UV light exposure at an intensity of ≥60 μW/cm 2 for 1 min, resulting in complete virus inactivation.

Original languageEnglish (US)
Pages (from-to)438-442
Number of pages5
JournalChinese Journal of Microbiology and Immunology
Volume29
Issue number5
DOIs
StatePublished - Dec 1 2009

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Ultraviolet Rays
Hepacivirus
Hot Temperature
Radiation
Viruses
Viral Load
Fluorescence
Baths
Virus Inactivation
Water
Limit of Detection
Cell Culture Techniques
Therapeutics

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Cite this

Study on the effects of heat and ultraviolet light radiation on hepatitis C virus JFH-1 strain inactivation. / Song, Hong Shuo; Shi, Shuang; Yan, Ling; Li, Kui; Zhuang, Hui.

In: Chinese Journal of Microbiology and Immunology, Vol. 29, No. 5, 01.12.2009, p. 438-442.

Research output: Contribution to journalArticle

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abstract = "Objective: To investigate the inactivating effect of heat and ultraviolet (UV) light on HCV JFH-1 strain using the cell culture system. Methods: The HCV JFH-1 virus stock, with an initial titer of 2.5 × 10 4 FFU/ml, was exposed in 56°C water bath or to UV light for varying durations of time for exploring their inactivating effects on the virus. The kinetics of virus titer reduction was determined by an indirect immuno-fluorescence assay (IFA). If the cells infected with the exposed virus stock were IFA negative after three blind passages, the virus stock was considered to be inactivated completely. Results: After incubation of the HCV JFH-1 virus stock (2.5 × 10 4 FFU/ml) in 56°C water bath for 10 min, 20 min and 30 min, the virus titers were reduced to 1.6 × 10 3 FFU/ml, 3.1 × 10 2 FFU/ml and 3.3 × 10 FFU/ml, respectively. The exposure of the virus stock to UV light (wavelength 253.7 nm, intensity ≥60 μW/cm 2, 30 cm below the UV lamp) for 15 s, 30 s and 45 s resulted in virus titer reduction to 1.0 × 10 3 FFU/ml, 1.1 × 10 2 FFU/ml and 2.7 × 10 FFU/ml, respectively. After 40 min incubation of the virus stock at 56°C, or 1 min exposure to UV light (wavelength 253.7 nm, intensity ≥60 μW/cm 2) the virus infectious titer was reduced below the detection limit of IFA, and the IFA was still negative even after three blind passages, indicating that the virus was inactivated completely. Conclusion: HCV is sensitive to heat and UV light treatment. For HCV JFH-1 virus stock containing 2.5 × 10 4 FFU/ml virus, heat treatment at 56°C for 40 min, or UV light exposure at an intensity of ≥60 μW/cm 2 for 1 min, resulting in complete virus inactivation.",
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