Superoxide scavengers do not prevent ischemia-induced alteration of cerebral vasodilation in piglets

Charles Leffler, Carolyn C. Thompson, William M. Armstead, Robert Mirro, Masaaki Shibata, David W. Busija

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Piglet brains generate superoxide during postischemic reperfusion, and topical application of activated oxygen species alters pial arteriolar responses. We investigated effects of pretreatment with scavengers of superoxide and H2O2 on ischemia-induced alterations of pial arteriolar responses in anesthetized newborn pigs. Four groups were studied: 1) time control, 2) untreated ischemia, 3) ischemia pretreated topically and systemically (conjugated to polyethylene glycol) with superoxide dis-mutase (SOD) and catalase, and 4) ischemia pretreated with Tiron. Pretreatment with SOD conjugated to polyethylene glycol alone during postischemic reperfusion effectively removed superoxide from its site of generation during postischemic reperfusion, but topical SOD was used also as insurance. Piglets were studied before and after 20 min of total cerebral ischemia caused by maintaining intracranial pressure above mean arterial pressure. As reported previously, before ischemia, hypercapnia and isoproterenol dilated pial arteries and arterioles and hypercapnia but not isoproterenol increased cortical periarachnoid cerebrospinal fluid 6-keto-prostaglandin F, measured as an index of cerebral cortical prostacyclin synthesis. After cerebral ischemia, pial arterioles did not dilate in response to hypercapnia and 6-keto-prostaglandin F did not increase, but dilation to isoproterenol was unchanged. The present study found that treatment with SOD/catalase or Tiron did not prevent loss of vasodilation to hypercapnia or the loss of hypercapnia-induced cerebral 6-keto-prostaglandin Fsynthesis after cerebral ischemia. The postischemic loss of cerebral vasodilation to hypercapnia does not appear to involve superoxide or a subsequent reduced form of oxygen.

Original languageEnglish (US)
Pages (from-to)164-170
Number of pages7
JournalPediatric Research
Volume33
Issue number2
DOIs
StatePublished - Jan 1 1993

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Vasodilation
Superoxides
Hypercapnia
Ischemia
Intramolecular Transferases
1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt
Brain Ischemia
Isoproterenol
Reperfusion
Arterioles
Catalase
Oxygen
Intracranial Pressure
Epoprostenol
Insurance
Prostaglandins
Cerebrospinal Fluid
Dilatation
Arterial Pressure
Swine

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health

Cite this

Superoxide scavengers do not prevent ischemia-induced alteration of cerebral vasodilation in piglets. / Leffler, Charles; Thompson, Carolyn C.; Armstead, William M.; Mirro, Robert; Shibata, Masaaki; Busija, David W.

In: Pediatric Research, Vol. 33, No. 2, 01.01.1993, p. 164-170.

Research output: Contribution to journalArticle

Leffler, Charles ; Thompson, Carolyn C. ; Armstead, William M. ; Mirro, Robert ; Shibata, Masaaki ; Busija, David W. / Superoxide scavengers do not prevent ischemia-induced alteration of cerebral vasodilation in piglets. In: Pediatric Research. 1993 ; Vol. 33, No. 2. pp. 164-170.
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