Suppression of oxidative stress by resveratrol after isometric contractions in gastrocnemius muscles of aged mice

Michael J. Ryan, Janna R. Jackson, Yanlei Hao, Courtney L. Williamson, Erinne R. Dabkowski, John M. Hollander, Stephen Alway

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

This study tested the hypothesis that resveratrol supplementation would lower oxidative stress in exercised muscles of aged mice. Young (3 months) and aged (27 months) C57BL/6 mice received a control or a 0.05% trans-resveratrol- supplemented diet for 10 days. After 7 days of dietary intervention, 20 maximal electrically evoked isometric contractions were obtained from the plantar flexors of one limb in anesthetized mice. Exercise was conducted for three consecutive days. Resveratrol supplementation blunted the exercise-induced increase in xanthine oxidase activity in muscles from young (25%) and aged (53%) mice. Resveratrol lowered H2O2 levels in control (13%) and exercised (38%) muscles from aged animals, reduced Nox4 protein in both control and exercised muscles of young (30%) and aged mice (40%), and increased the ratio of reduced glutathione to oxidized glutathione in exercised muscles from young (38%) and aged (135%) mice. Resveratrol prevented the increase in lipid oxidation, increased catalase activity, and increased MnSOD activity in exercised muscles from aged mice. These data show that dietary resveratrol suppresses muscle indicators of oxidative stress in response to isometric contractions in aged mice.

Original languageEnglish (US)
Pages (from-to)815-831
Number of pages17
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume65 A
Issue number8
DOIs
StatePublished - Aug 1 2010

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Isometric Contraction
Skeletal Muscle
Oxidative Stress
Muscles
Glutathione Disulfide
Xanthine Oxidase
resveratrol
Inbred C57BL Mouse
Catalase
Glutathione
Extremities
Diet
Lipids

All Science Journal Classification (ASJC) codes

  • Aging
  • Geriatrics and Gerontology

Cite this

Suppression of oxidative stress by resveratrol after isometric contractions in gastrocnemius muscles of aged mice. / Ryan, Michael J.; Jackson, Janna R.; Hao, Yanlei; Williamson, Courtney L.; Dabkowski, Erinne R.; Hollander, John M.; Alway, Stephen.

In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences, Vol. 65 A, No. 8, 01.08.2010, p. 815-831.

Research output: Contribution to journalArticle

Ryan, Michael J. ; Jackson, Janna R. ; Hao, Yanlei ; Williamson, Courtney L. ; Dabkowski, Erinne R. ; Hollander, John M. ; Alway, Stephen. / Suppression of oxidative stress by resveratrol after isometric contractions in gastrocnemius muscles of aged mice. In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences. 2010 ; Vol. 65 A, No. 8. pp. 815-831.
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