Survey of the distribution of a newly characterized receptor for advanced glycation end products in tissues

J. Brett, A. M. Schmidt, Du Yan Shi Du Yan, Shan Zou Yu Shan Zou, E. Weidman, D. Pinsky, R. Nowygrod, M. Neeper, C. Przysiecki, A. Shaw, A. Migheli, David Stern

Research output: Contribution to journalArticle

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Abstract

Advanced glycation end products (AGEs), the final products of nonenzymatic glycation and oxidation of proteins, are found in the plasma and accumulate in the tissues during aging and at an accelerated rate in diabetes. A novel integral membrane protein, termed receptor for AGE (RAGE), forms a central part of the cell surface binding site for AGEs. Using monospecific, polyclonal antibody raised to human recombinant and bovine RAGE, immunostaining of bovine tissues showed RAGE in the vasculature, endothelium, and smooth muscle cells and in mononuclear cells in the tissues. Consistent with these data, RAGE antigen and mRNA were identified in cultured bovine endothelium, vascular smooth muscle, and monocyte-derived macrophages. RAGE antigen was also visualized in bovine cardiac myocytes as well as in cultures of neonatal rat cardiac myocytes and in neural tissue where motor neurons, peripheral nerves, and a population of cortical neurons were positive. In situ hybridization confirmed the presence of RAGE mRNA in the tissues, and studies with rat PC12 pheochromocytes indicated that they provide a neuronal- related cell culture model for examining RAGE expression. Pathological studies of human atherosclerotic plaques showed infiltration of RAGE- expressing cells in the expanded intima. These results indicate that RAGE is present in multiple tissues and suggest the potential relevance of AGE-RAGE interactions for modulating properties of the vasculature as well as neural and cardiac function, prominent areas of involvement in diabetes and in the normal aging process.

Original languageEnglish (US)
Pages (from-to)1699-1712
Number of pages14
JournalAmerican Journal of Pathology
Volume143
Issue number6
StatePublished - Dec 1 1993
Externally publishedYes

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Antigen Receptors
Cardiac Myocytes
Endothelium
Advanced Glycosylation End Product-Specific Receptor
Surveys and Questionnaires
Messenger RNA
Advanced Glycosylation End Products
Motor Neurons
Atherosclerotic Plaques
Vascular Smooth Muscle
Peripheral Nerves
Smooth Muscle Myocytes
In Situ Hybridization
Membrane Proteins
Cell Culture Techniques
Macrophages
Binding Sites
Neurons
Antibodies
Population

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Brett, J., Schmidt, A. M., Shi Du Yan, D. Y., Yu Shan Zou, S. Z., Weidman, E., Pinsky, D., ... Stern, D. (1993). Survey of the distribution of a newly characterized receptor for advanced glycation end products in tissues. American Journal of Pathology, 143(6), 1699-1712.

Survey of the distribution of a newly characterized receptor for advanced glycation end products in tissues. / Brett, J.; Schmidt, A. M.; Shi Du Yan, Du Yan; Yu Shan Zou, Shan Zou; Weidman, E.; Pinsky, D.; Nowygrod, R.; Neeper, M.; Przysiecki, C.; Shaw, A.; Migheli, A.; Stern, David.

In: American Journal of Pathology, Vol. 143, No. 6, 01.12.1993, p. 1699-1712.

Research output: Contribution to journalArticle

Brett, J, Schmidt, AM, Shi Du Yan, DY, Yu Shan Zou, SZ, Weidman, E, Pinsky, D, Nowygrod, R, Neeper, M, Przysiecki, C, Shaw, A, Migheli, A & Stern, D 1993, 'Survey of the distribution of a newly characterized receptor for advanced glycation end products in tissues', American Journal of Pathology, vol. 143, no. 6, pp. 1699-1712.
Brett J, Schmidt AM, Shi Du Yan DY, Yu Shan Zou SZ, Weidman E, Pinsky D et al. Survey of the distribution of a newly characterized receptor for advanced glycation end products in tissues. American Journal of Pathology. 1993 Dec 1;143(6):1699-1712.
Brett, J. ; Schmidt, A. M. ; Shi Du Yan, Du Yan ; Yu Shan Zou, Shan Zou ; Weidman, E. ; Pinsky, D. ; Nowygrod, R. ; Neeper, M. ; Przysiecki, C. ; Shaw, A. ; Migheli, A. ; Stern, David. / Survey of the distribution of a newly characterized receptor for advanced glycation end products in tissues. In: American Journal of Pathology. 1993 ; Vol. 143, No. 6. pp. 1699-1712.
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AU - Pinsky, D.

AU - Nowygrod, R.

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