Synaptophysin immunohistochemistry densitometry measurement in resected human hippocampus

Implication for the etiology of hippocampal sclerosis

Keith G. Davies, John B. Schweitzer, Mark R. Looney, Andrew J. Bush, F Dohan, Bruce P. Hermann

Research output: Contribution to journalArticle

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Abstract

Synaptophysin (SY) is a protein expressed at presynaptic vesicles. SY immunohistochemistry (IHC) was undertaken in surgically resected hippocampal specimens from 25 patients with intractable epilepsy. All were investigated with chronic ictal EEG videotelemetry, which showed a temporal onset in each case, and all had normal magnetic resonance imaging (MRI). The density of reaction product of SY IHC was used to generate optical density (OD) measurements as an estimate of synaptic density in CA1 and CA4 fields (ODCA1 and ODCA4), and inner third and outer two-thirds of the molecular layer of the dentate gyrus (ODIML and ODOML). There was significant correlation between ODCA1 (r = 0.619, P = 0.001) and ODCA4 (r = 0.639, P = 0.001) and onset age of epilepsy. There was no correlation between ODCA1 and duration of epilepsy. There was correlation between ODCA4 and duration (r = -0.412, P = 0.041), but partial correlations showed no significant correlation between ODCA4 and duration when controlling for onset, although correlation between ODCA4 and onset when controlling for duration remained significant (r = 0.536, P < 0.01). Univariate ANOVA revealed onset age to be the only predictor of ODCA1 and ODCA4. Chronological age and duration were not predictors. There was no correlation between ODIML or ODOML and either onset age or duration. ODIML and ODOML were not predicted by onset age, duration or chronological age. These findings support the hypothesis that the major cause of hippocampal sclerosis is an age specific insult rather than the result of repeated seizures.

Original languageEnglish (US)
Pages (from-to)335-344
Number of pages10
JournalEpilepsy Research
Volume32
Issue number3
DOIs
StatePublished - Nov 1 1998

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Synaptophysin
Densitometry
Sclerosis
Age of Onset
Hippocampus
Immunohistochemistry
Epilepsy
Dentate Gyrus
Electroencephalography
Analysis of Variance
Seizures
Stroke
Magnetic Resonance Imaging
Proteins

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology

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Synaptophysin immunohistochemistry densitometry measurement in resected human hippocampus : Implication for the etiology of hippocampal sclerosis. / Davies, Keith G.; Schweitzer, John B.; Looney, Mark R.; Bush, Andrew J.; Dohan, F; Hermann, Bruce P.

In: Epilepsy Research, Vol. 32, No. 3, 01.11.1998, p. 335-344.

Research output: Contribution to journalArticle

Davies, Keith G. ; Schweitzer, John B. ; Looney, Mark R. ; Bush, Andrew J. ; Dohan, F ; Hermann, Bruce P. / Synaptophysin immunohistochemistry densitometry measurement in resected human hippocampus : Implication for the etiology of hippocampal sclerosis. In: Epilepsy Research. 1998 ; Vol. 32, No. 3. pp. 335-344.
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