Syngeneic immune response to rat tracheal epithelial cells transformed in vitro by n-methyl-n-nitro-n-nitrosoguanidine

G. R. Braslawsky, V. Steele, Stephen Kennel, P. Nettesheim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Two cell lines (2-10-1 and 8-10-2) derived by exposure to primary tracheal explants to MNNG in vitro were not tumorigenic in syngeneic F-344 rats or athymic BALB/c (nu/nu) mice at early passage, but became tumorigenic at late passage. These cell lines are therefore suited to study the expression of neoantigens during neoplastic development. Transplantation resistance to late-passage, tumorigenic cells was indicated in syngeneic rats using an immunization protocol of repeated cell inoculation and tumour ablation. Spleen cells from such animals were reactive in 20h microcytotoxicity assays against neoplastic cell lines, but unreactive to normal tracheal epithelial cells. Similarly, immune spleen cells co-cultivated in vitro for 6 days with irradiated neoplastic cell lines before assay for microcytotoxicity were strongly reactive, whereas co-cultivation with normal epithelial cells did not stimulate reactivity. Antibody to these neoplastic cell lines was demonstrated in sera of tumour-resistant rats by an indirect radiolabelled-antibody binding test and by indirect immunofluorescence. There was no significant binding to normal tracheal epithelial cell outgrowths.

Original languageEnglish (US)
Pages (from-to)247-257
Number of pages11
JournalBritish Journal of Cancer
Volume44
Issue number2
DOIs
StatePublished - Jan 1 1981
Externally publishedYes

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Nitrosoguanidines
Epithelial Cells
Cell Line
Spleen
Nude Rats
Methylnitronitrosoguanidine
Indirect Fluorescent Antibody Technique
Immunization
Neoplasms
Transplantation
In Vitro Techniques
Antibodies
Serum

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Syngeneic immune response to rat tracheal epithelial cells transformed in vitro by n-methyl-n-nitro-n-nitrosoguanidine. / Braslawsky, G. R.; Steele, V.; Kennel, Stephen; Nettesheim, P.

In: British Journal of Cancer, Vol. 44, No. 2, 01.01.1981, p. 247-257.

Research output: Contribution to journalArticle

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