Synthesis and anti-staphylococcal activity of TiO2 nanoparticles and nanowires in ex vivo porcine skin model

Namrata Nataraj, G. S. Anjusree, Asha Anish Madhavan, P. Priyanka, Deepthi Sankar, Nisha Nair, S. V. Lakshmi, R. Jayakumar, Avinash Balakrishnan, Raja Biswas

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Staphylococcus aureus is one of the major causes of skin and soft tissue infections. In this study we compared the antimicrobial activity of two different TiO2 nanoformulations against Staphylococcus aureus. We synthesized TiO2 nanoparticles of ∼80 nm diameter and TiO 2 nanowires of ∼100 nm diameter. Both nanoformulations possess anti-microbial activity; were non-hemolytic and cytocompatible. However, the anti-staphylococcal activity of TiO2 nanowires was better than the nanoparticles. In broth culture, growth of S. aureus was only partially inhibited by 2% and 4 wt% TiO2 nanoparticles and completely inhibited by TiO2 nanowires till 24 h. TiO2 nanowires treated S. aureus cells exhibits diminished membrane potential than nanoparticle treated cells. The anti-microbial properties of both TiO2 nanoformulations were validated using ex vivo porcine skin model which supplements the in vitro assays. Anti-bacterial activity of the TiO2 nanowires were also validated against multi drug resistant pathogenic strains of S. aureus, showing the clinical potency of the TiO2 nanowires compared to its nanoparticles.

Original languageEnglish (US)
Pages (from-to)864-870
Number of pages7
JournalJournal of Biomedical Nanotechnology
Volume10
Issue number5
DOIs
StatePublished - Jan 1 2014

Fingerprint

Nanowires
Nanoparticles
Skin
Swine
Staphylococcus aureus
Soft Tissue Infections
Membrane Potentials
Assays
Cells
Tissue
Membranes
Growth
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

Cite this

Nataraj, N., Anjusree, G. S., Madhavan, A. A., Priyanka, P., Sankar, D., Nair, N., ... Biswas, R. (2014). Synthesis and anti-staphylococcal activity of TiO2 nanoparticles and nanowires in ex vivo porcine skin model. Journal of Biomedical Nanotechnology, 10(5), 864-870. https://doi.org/10.1166/jbn.2014.1756

Synthesis and anti-staphylococcal activity of TiO2 nanoparticles and nanowires in ex vivo porcine skin model. / Nataraj, Namrata; Anjusree, G. S.; Madhavan, Asha Anish; Priyanka, P.; Sankar, Deepthi; Nair, Nisha; Lakshmi, S. V.; Jayakumar, R.; Balakrishnan, Avinash; Biswas, Raja.

In: Journal of Biomedical Nanotechnology, Vol. 10, No. 5, 01.01.2014, p. 864-870.

Research output: Contribution to journalArticle

Nataraj, N, Anjusree, GS, Madhavan, AA, Priyanka, P, Sankar, D, Nair, N, Lakshmi, SV, Jayakumar, R, Balakrishnan, A & Biswas, R 2014, 'Synthesis and anti-staphylococcal activity of TiO2 nanoparticles and nanowires in ex vivo porcine skin model', Journal of Biomedical Nanotechnology, vol. 10, no. 5, pp. 864-870. https://doi.org/10.1166/jbn.2014.1756
Nataraj, Namrata ; Anjusree, G. S. ; Madhavan, Asha Anish ; Priyanka, P. ; Sankar, Deepthi ; Nair, Nisha ; Lakshmi, S. V. ; Jayakumar, R. ; Balakrishnan, Avinash ; Biswas, Raja. / Synthesis and anti-staphylococcal activity of TiO2 nanoparticles and nanowires in ex vivo porcine skin model. In: Journal of Biomedical Nanotechnology. 2014 ; Vol. 10, No. 5. pp. 864-870.
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