Synthesis and application of 13C enriched phenylisothiocyanate as a 13C NMR protein probe

Jeffrey J. Ares, John W. Fowble, Thomas G. Urchek, Duane Miller

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Phenylisothiocyanate, enriched with 13C at the isothiocyanate carbon, has been synthesized and utilized as a 13C NMR probe of proteins for the first time. The reagent has been used to label the amino groups of oxidized glutathione, and the resulting 13C NMR spectrum shows a prominent thiocarbonyl peak after a single NMR scan. The reagent is also capable of differentiating amino groups on the insulin molecule with distinct peaks corresponding to the amino groups on the A and B chains of insulin. This study illustrates the potential of using a new 13C label to functionalize amino groups of proteins and to study the labeled proteins with 13C NMR.

Original languageEnglish (US)
Pages (from-to)1064-1071
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume142
Issue number3
DOIs
StatePublished - Feb 13 1987

Fingerprint

Nuclear magnetic resonance
Insulin
Labels
Proteins
Glutathione Disulfide
Carbon
Molecules
phenylisothiocyanate
Carbon-13 Magnetic Resonance Spectroscopy
isothiocyanic acid

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Synthesis and application of 13C enriched phenylisothiocyanate as a 13C NMR protein probe. / Ares, Jeffrey J.; Fowble, John W.; Urchek, Thomas G.; Miller, Duane.

In: Biochemical and Biophysical Research Communications, Vol. 142, No. 3, 13.02.1987, p. 1064-1071.

Research output: Contribution to journalArticle

Ares, Jeffrey J. ; Fowble, John W. ; Urchek, Thomas G. ; Miller, Duane. / Synthesis and application of 13C enriched phenylisothiocyanate as a 13C NMR protein probe. In: Biochemical and Biophysical Research Communications. 1987 ; Vol. 142, No. 3. pp. 1064-1071.
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