Synthesis and release of interleukin 1 by reoxygenated human mononuclear phagocytes

S. Koga, S. Ogawa, K. Kuwabara, J. Brett, J. A. Leavy, J. Ryan, Y. Koga, J. Plocinski, W. Benjamin, D. K. Burns, David Stern

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Abstract

To examine the possible involvement of cytokines in reperfusion injury, we have studied production of IL-1 by human vascular cells, including smooth muscle and mononuclear phagocytes. Exposure of cells to hypoxia (pO2 ≃ 14 torr) followed by reoxygenation led to significant release of IL-1 only from the mononuclear phagocytes. Elaboration of IL-1 was dependent on the oxygen tension and duration of hypoxia (optimal at lower pO2s, ≃ 14-20 torr, and after 9 h), as well as the time in reoxygenation (maximal IL-1 release at 6-9 h). Although a period of hypoxia was necessary for subsequent IL-1 production during reoxygenation of either peripheral blood monocytes or cultured monocyte-derived macrophages, no IL-1 release occurred during the hypoxic exposure. IL-1 released during reoxygenation was newly synthesized, and its production was triggered by the generation of oxygen free radicals, as it could be blocked by the addition of either allopurinol or free radical scavengers to cultures and could be stimulated in part by low concentrations of hydrogen peroxide or xanthine/xanthine oxidase. The potential pathophysiological effects of IL-1-containing supernatants from reoxygenated macrophages was shown by their induction of endothelial tissue factor and enhancement of endothelial adhesiveness for neutrophils, both of which could be blocked by anti-IL-1 antibody. The relevance of IL-1 to hypoxia/reoxygenation in vivo was suggested by the presence of circulating nanogram amounts of this cytokine in the plasma of mice during the reoxygenation period following a hypoxia.

Original languageEnglish (US)
Pages (from-to)1007-1015
Number of pages9
JournalJournal of Clinical Investigation
Volume90
Issue number3
DOIs
StatePublished - Jan 1 1992

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Phagocytes
Interleukin-1
Macrophages
Cytokines
Cell Hypoxia
Adhesiveness
Free Radical Scavengers
Allopurinol
Xanthine
Xanthine Oxidase
Thromboplastin
Reperfusion Injury
Hydrogen Peroxide
Free Radicals
Smooth Muscle Myocytes
Endothelium
Blood Vessels
Monocytes
Reactive Oxygen Species
Neutrophils

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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Synthesis and release of interleukin 1 by reoxygenated human mononuclear phagocytes. / Koga, S.; Ogawa, S.; Kuwabara, K.; Brett, J.; Leavy, J. A.; Ryan, J.; Koga, Y.; Plocinski, J.; Benjamin, W.; Burns, D. K.; Stern, David.

In: Journal of Clinical Investigation, Vol. 90, No. 3, 01.01.1992, p. 1007-1015.

Research output: Contribution to journalArticle

Koga, S, Ogawa, S, Kuwabara, K, Brett, J, Leavy, JA, Ryan, J, Koga, Y, Plocinski, J, Benjamin, W, Burns, DK & Stern, D 1992, 'Synthesis and release of interleukin 1 by reoxygenated human mononuclear phagocytes', Journal of Clinical Investigation, vol. 90, no. 3, pp. 1007-1015. https://doi.org/10.1172/JCI115913
Koga, S. ; Ogawa, S. ; Kuwabara, K. ; Brett, J. ; Leavy, J. A. ; Ryan, J. ; Koga, Y. ; Plocinski, J. ; Benjamin, W. ; Burns, D. K. ; Stern, David. / Synthesis and release of interleukin 1 by reoxygenated human mononuclear phagocytes. In: Journal of Clinical Investigation. 1992 ; Vol. 90, No. 3. pp. 1007-1015.
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