Synthesis, assembly, and turnover of α and β-erythroid and nonerythroid spectrins in rat hippocampal neurons

José Sangerman, Anthony L. Gard, Arvind Shah, Steven Goodman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The synthesis and turnover of α-erythroid, β-erythroid, α-nonerythroid and β-nonerythroid spectrins was investigated in cultured rat hippocampal neurons. [35S]methionine and subunit specific antibodies were used to label and immunoprecipitate newly synthesized spectrins in 12- to 14-day-old cultures. Synthesis experiments, performed under normal resting conditions, showed that the ratio of newly synthesized α-erythroid/β-erythroid and α-nonerythroid/β-nonerythroid spectrins is 1/1 (mol/mol) both in the soluble and insoluble fractions. Soluble and insoluble α and β erythroid spectrin turn over rapidly (half-life=16-24 min). Soluble nonerythroid α-spectrin (half-life=80 min) and β spectrin (half-life=53 min) turn over more slowly than their insoluble counterparts (30-34 min). The nonerythroid α spectrin turnover was significantly different (p<0.05) from the other measurements except for nonerythroid β spectrin, indicating that these subunits are protected from rapid proteolytic degradation until they are assembled in the membrane skeleton. Copyright (C) 1999 Elsevier Science.

Original languageEnglish (US)
Pages (from-to)128-138
Number of pages11
JournalBrain Research
Volume849
Issue number1-2
DOIs
StatePublished - Dec 4 1999
Externally publishedYes

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Spectrin
Neurons
Half-Life
Skeleton
Methionine
Membranes
Antibodies

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Synthesis, assembly, and turnover of α and β-erythroid and nonerythroid spectrins in rat hippocampal neurons. / Sangerman, José; Gard, Anthony L.; Shah, Arvind; Goodman, Steven.

In: Brain Research, Vol. 849, No. 1-2, 04.12.1999, p. 128-138.

Research output: Contribution to journalArticle

Sangerman, José ; Gard, Anthony L. ; Shah, Arvind ; Goodman, Steven. / Synthesis, assembly, and turnover of α and β-erythroid and nonerythroid spectrins in rat hippocampal neurons. In: Brain Research. 1999 ; Vol. 849, No. 1-2. pp. 128-138.
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