Synthesis of cyclopropane fatty acids in isolated bacterial membranes

G. Stanley Cox, Edwin Thomas, H. R. Kaback, Herbert Weissbach

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Isolated E. coli membrane vesicles can synthesize cyclopropane fatty acids from S-adenosylmethionine using endogenous membrane phospholipid as the lipidsubstrate. The major methylated products are methylenehexadecanoic acid and methyleneoctadecenoic acid esterified to phosphatidylethanolamine. The membrane system is slightly stimulated by sodium dodecyl sulfate at low concentrations but is inhibited by neutral surfactants. The reaction is inhibited by phospholipase C and phospholipase A. The Arrhenius plot for the enzyme reaction is discontinuous over the temperature range 0-35 °C.

Original languageEnglish (US)
Pages (from-to)667-676
Number of pages10
JournalArchives of Biochemistry and Biophysics
Volume158
Issue number2
DOIs
StatePublished - Jan 1 1973
Externally publishedYes

Fingerprint

Membranes
S-Adenosylmethionine
Arrhenius plots
Phospholipases A
Type C Phospholipases
Surface-Active Agents
Sodium Dodecyl Sulfate
Escherichia coli
Phospholipids
Temperature
Acids
Enzymes
cyclopropane fatty acids
phosphatidylethanolamine
methylene hexadecanoic acid

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Synthesis of cyclopropane fatty acids in isolated bacterial membranes. / Cox, G. Stanley; Thomas, Edwin; Kaback, H. R.; Weissbach, Herbert.

In: Archives of Biochemistry and Biophysics, Vol. 158, No. 2, 01.01.1973, p. 667-676.

Research output: Contribution to journalArticle

Cox, G. Stanley ; Thomas, Edwin ; Kaback, H. R. ; Weissbach, Herbert. / Synthesis of cyclopropane fatty acids in isolated bacterial membranes. In: Archives of Biochemistry and Biophysics. 1973 ; Vol. 158, No. 2. pp. 667-676.
@article{e01b0def602349e190bdd9005fc6bc5f,
title = "Synthesis of cyclopropane fatty acids in isolated bacterial membranes",
abstract = "Isolated E. coli membrane vesicles can synthesize cyclopropane fatty acids from S-adenosylmethionine using endogenous membrane phospholipid as the lipidsubstrate. The major methylated products are methylenehexadecanoic acid and methyleneoctadecenoic acid esterified to phosphatidylethanolamine. The membrane system is slightly stimulated by sodium dodecyl sulfate at low concentrations but is inhibited by neutral surfactants. The reaction is inhibited by phospholipase C and phospholipase A. The Arrhenius plot for the enzyme reaction is discontinuous over the temperature range 0-35 °C.",
author = "Cox, {G. Stanley} and Edwin Thomas and Kaback, {H. R.} and Herbert Weissbach",
year = "1973",
month = "1",
day = "1",
doi = "10.1016/0003-9861(73)90559-6",
language = "English (US)",
volume = "158",
pages = "667--676",
journal = "Archives of Biochemistry and Biophysics",
issn = "0003-9861",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Synthesis of cyclopropane fatty acids in isolated bacterial membranes

AU - Cox, G. Stanley

AU - Thomas, Edwin

AU - Kaback, H. R.

AU - Weissbach, Herbert

PY - 1973/1/1

Y1 - 1973/1/1

N2 - Isolated E. coli membrane vesicles can synthesize cyclopropane fatty acids from S-adenosylmethionine using endogenous membrane phospholipid as the lipidsubstrate. The major methylated products are methylenehexadecanoic acid and methyleneoctadecenoic acid esterified to phosphatidylethanolamine. The membrane system is slightly stimulated by sodium dodecyl sulfate at low concentrations but is inhibited by neutral surfactants. The reaction is inhibited by phospholipase C and phospholipase A. The Arrhenius plot for the enzyme reaction is discontinuous over the temperature range 0-35 °C.

AB - Isolated E. coli membrane vesicles can synthesize cyclopropane fatty acids from S-adenosylmethionine using endogenous membrane phospholipid as the lipidsubstrate. The major methylated products are methylenehexadecanoic acid and methyleneoctadecenoic acid esterified to phosphatidylethanolamine. The membrane system is slightly stimulated by sodium dodecyl sulfate at low concentrations but is inhibited by neutral surfactants. The reaction is inhibited by phospholipase C and phospholipase A. The Arrhenius plot for the enzyme reaction is discontinuous over the temperature range 0-35 °C.

UR - http://www.scopus.com/inward/record.url?scp=0015861526&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015861526&partnerID=8YFLogxK

U2 - 10.1016/0003-9861(73)90559-6

DO - 10.1016/0003-9861(73)90559-6

M3 - Article

VL - 158

SP - 667

EP - 676

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

IS - 2

ER -