Synthesis of heat shock proteins in rat brain cortex after transient ischemia

G. A. Dienel, M. Kiessling, Michael Jacewicz, W. A. Pulsinelli

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Cell-free protein synthesis and two-dimensional gel autoradiography were used to characterize early postischemic protein synthesis in rat neocortex. Severe forebrain ischemia was induced for 30 min (fourvessel occlusion model) and followed by 3 h of recirculation. Polysomes were isolated from the cerebral cortex, translated in vitro in a reticulocyte system, and analyzed by two-dimensional gel electrophoresis. The translation products of postischemic polysomes included a major new protein family (70 kDa) with multiple isoelectric variants that was found to comigrate with the 68- to 70-kDa 'heat shock' protein synthesized from polysomes of hyperthermic rats. Two other stress proteins (93 and 110 kDa) also appeared to be synthesized in increased amounts after ischemia. A complement of proteins that was indistinguishable from that of controls was also synthesized after ischemia, indicating that messenger ribonucleic acid coding for most brain proteins is preserved after ischemia and is bound to polysomes.

Original languageEnglish (US)
Pages (from-to)505-510
Number of pages6
JournalJournal of Cerebral Blood Flow and Metabolism
Volume6
Issue number4
DOIs
StatePublished - Jan 1 1986

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Polyribosomes
Heat-Shock Proteins
Ischemia
Brain
Proteins
HSP70 Heat-Shock Proteins
Reticulocytes
Neocortex
Electrophoresis, Gel, Two-Dimensional
Prosencephalon
Autoradiography
Cerebral Cortex
Complement System Proteins
Gels
RNA

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Synthesis of heat shock proteins in rat brain cortex after transient ischemia. / Dienel, G. A.; Kiessling, M.; Jacewicz, Michael; Pulsinelli, W. A.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 6, No. 4, 01.01.1986, p. 505-510.

Research output: Contribution to journalArticle

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