Synthesis of viral DNA in the cytoplasm of duck embryo fibroblasts and in enucleated cells after infection by avian sarcoma virus

H. E. Varmus, Ramareddy Guntaka, W. J.W. Fan, S. Heasley, J. M. Bishop

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Two lines of evidence indicate that synthesis of viral DNA occurs in the cytoplasm of duck embryo fibroblasts infected with avian sarcoma virus: viral DNA is detected first in the cytoplasmic fraction of infected cells and subsequently in the nuclear fraction; and viral DNA is synthetized at a normal rate in cells infected after enucleation with cytochalasin B. The presence of viral DNA in the cytoplasmic fraction is not a consequence of leakage of newly synthesized viral DNA from the nucleus, since it remains in nuclear fractions late after infection when integration of viral DNA into the host genome is inhibited by ethidium bromide.

Original languageEnglish (US)
Pages (from-to)3874-3878
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume71
Issue number10
DOIs
StatePublished - Dec 1 1974

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Avian Sarcoma Viruses
Ducks
Viral DNA
Cytoplasm
Embryonic Structures
Fibroblasts
Infection
Cytochalasin B
Ethidium
Genome

All Science Journal Classification (ASJC) codes

  • General

Cite this

Synthesis of viral DNA in the cytoplasm of duck embryo fibroblasts and in enucleated cells after infection by avian sarcoma virus. / Varmus, H. E.; Guntaka, Ramareddy; Fan, W. J.W.; Heasley, S.; Bishop, J. M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 71, No. 10, 01.12.1974, p. 3874-3878.

Research output: Contribution to journalArticle

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