Systemic elevation of interleukin-15 in vivo promotes apoptosis in skeletal muscles of young adult and aged rats

Emidio E. Pistilli, Stephen Alway

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In this study, we tested the hypothesis that systemic elevation of IL-15 would attenuate apoptosis in skeletal muscles of aged rats. IL-15 was administered to young adult (n = 6) and aged (n = 6) rats for 14 days. Apoptosis was quantified using an ELISA assay and verified through TUNEL staining of muscle sections. As expected, apoptosis was greater in muscles from aged control rats, compared to age-matched control. Apoptosis was also greater in the muscles from young adult and aged rats treated with IL-15. These increases in apoptosis were associated with decreases in muscle mass of IL-15 treated rats. These data do not support our initial hypothesis and suggest that systemic elevation of IL-15 promotes apoptosis in skeletal muscle. The proposed anti-apoptotic property of IL-15 may be specific to cell-type and/or the degree of muscle pathology present; however, additional research is required to more clearly decipher its role in skeletal muscle.

Original languageEnglish (US)
Pages (from-to)20-24
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume373
Issue number1
DOIs
StatePublished - Aug 15 2008
Externally publishedYes

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Interleukin-15
Muscle
Rats
Young Adult
Skeletal Muscle
Apoptosis
Muscles
Rat control
In Situ Nick-End Labeling
Pathology
Enzyme-Linked Immunosorbent Assay
Staining and Labeling
Assays
Research

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Systemic elevation of interleukin-15 in vivo promotes apoptosis in skeletal muscles of young adult and aged rats. / Pistilli, Emidio E.; Alway, Stephen.

In: Biochemical and Biophysical Research Communications, Vol. 373, No. 1, 15.08.2008, p. 20-24.

Research output: Contribution to journalArticle

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