Tandem mass spectrometry of peptides. III—differentiation between leucine and isoleucine based on neutral losses

Nicole L. Squire, Sarka Beranova, Chrys Wesdemiotis

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The neutral species generated during the collisionally activated dissociation (CAD) of the protonated dipeptides Ala–Leu, Ala–Ile, Leu–Ala and Ile–Ala are post‐ionized by collision and detected in neutral fragment‐reionization (+NfR+) mass spectra. These spectra are dominated by fragment ions diagnostic for the C‐terminal amino acid, as confirmed by comparison with reference collision‐induced dissociative ionization (CIDI) mass spectra of authentic Ala, Leu and Ile. CIDI of Leu and Ile yields distinct and structurally indicative products. These are also observed upon +NfR+ of [Ala–Leu]H+ and [Ala–Ile]H+, respectively, thus allowing one to deduce the correct sequence. In contrast, [Leu–Ala]H+ and [Ile–Ala]H+, which possess identical C‐termini, form no differentiating neutral losses upon CAD.

Original languageEnglish (US)
Pages (from-to)1429-1434
Number of pages6
JournalJournal of Mass Spectrometry
Volume30
Issue number10
DOIs
StatePublished - Jan 1 1995

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Isoleucine
Leucine
Ionization
Mass spectrometry
Peptides
Dipeptides
Ions
Amino Acids

All Science Journal Classification (ASJC) codes

  • Spectroscopy

Cite this

Tandem mass spectrometry of peptides. III—differentiation between leucine and isoleucine based on neutral losses. / Squire, Nicole L.; Beranova, Sarka; Wesdemiotis, Chrys.

In: Journal of Mass Spectrometry, Vol. 30, No. 10, 01.01.1995, p. 1429-1434.

Research output: Contribution to journalArticle

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