Tannic acid induces endoplasmic reticulum stress-mediated apoptosis in prostate cancer

Prashanth K.B. Nagesh, Elham Hatami, Pallabita Chowdhury, Vivek K. Kashyap, Sheema Khan, Bilal Hafeez, Subhash Chauhan, Meena Jaggi, Murali Yallapu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Endoplasmic reticulum (ER) stress is an intriguing target with significant clinical importance in chemotherapy. Interference with ER functions can lead to the accumulation of unfolded proteins, as detected by transmembrane sensors that instigate the unfolded protein response (UPR). Therefore, controlling induced UPR via ER stress with natural compounds could be a novel therapeutic strategy for the management of prostate cancer. Tannic acid (a naturally occurring polyphenol) was used to examine the ER stress mediated UPR pathway in prostate cancer cells. Tannic acid treatment inhibited the growth, clonogenic, invasive, and migratory potential of prostate cancer cells. Tannic acid demonstrated activation of ER stress response (Protein kinase R-like endoplasmic reticulum kinase (PERK) and inositol requiring enzyme 1 (IRE1)) and altered its regulatory proteins (ATF4, Bip, and PDI) expression. Tannic acid treatment affirmed upregulation of apoptosis-associated markers (Bak, Bim, cleaved caspase 3, and cleaved PARP), while downregulation of pro-survival proteins (Bcl-2 and Bcl-xL). Tannic acid exhibited elevated G1 population, due to increase in p18INK4C and p21WAF1/CIP1 expression, while cyclin D1 expression was inhibited. Reduction of MMP2 and MMP9, and reinstated E-cadherin signifies the anti-metastatic potential of this compound. Altogether, these results demonstrate that tannic acid can promote apoptosis via the ER stress mediated UPR pathway, indicating a potential candidate for cancer treatment.

Original languageEnglish (US)
Article number68
JournalCancers
Volume10
Issue number3
DOIs
StatePublished - Mar 7 2018

Fingerprint

Endoplasmic Reticulum Stress
Tannins
Unfolded Protein Response
Prostatic Neoplasms
Apoptosis
Endoplasmic Reticulum
Protein Unfolding
Cyclin D1
Polyphenols
Inositol
Cadherins
Therapeutics
Heat-Shock Proteins
Caspase 3
Protein Kinases
Proteins
Up-Regulation
Down-Regulation
Drug Therapy
Enzymes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Tannic acid induces endoplasmic reticulum stress-mediated apoptosis in prostate cancer. / Nagesh, Prashanth K.B.; Hatami, Elham; Chowdhury, Pallabita; Kashyap, Vivek K.; Khan, Sheema; Hafeez, Bilal; Chauhan, Subhash; Jaggi, Meena; Yallapu, Murali.

In: Cancers, Vol. 10, No. 3, 68, 07.03.2018.

Research output: Contribution to journalArticle

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