Tannic acid-lung fluid assemblies promote interaction and delivery of drugs to lung cancer cells

Elham Hatami, Prashanth K.B. Nagesh, Pallabita Chowdhury, Subhash Chauhan, Meena Jaggi, Amali Samarasinghe, Murali Yallapu

Research output: Contribution to journalArticle

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Abstract

Lung cancer (LC) is one of the leading causes of death in both men and women in the United States. Tannic acid (TA), a water-soluble polyphenol, exhibits a wide range of biological activities. TA has received much attention as a promising compound in the biomaterial and drug delivery fields. Lung fluid (LF) is a major barrier for distribution of drugs to the lungs. Therefore, the purpose of this study was to examine TA interaction with LF for effective delivery of anti-cancer drug molecules via pulmonary delivery. The extent of adsorption of LF proteins by TA was revealed by fluorescence quenching in fluorescence spectroscopy. The presence of LF in TA-LF complexes was noticed by the presence of protein peaks at 1653 cm−1. Both protein dot and SDS-PAGE analysis confirmed LF protein complexation at all TA concentrations employed. A stable particle TA-LF complex formation was observed through transmission electron microscopy (TEM) analysis. The complexation pattern measured by dynamic light scattering (DLS) indicated that it varies depending on the pH of the solutions. The degree of LF presence in TA-LF complexes signifies its interactive behavior in LC cell lines. Such superior interaction offered an enhanced anti-cancer activity of drugs encapsulated in TA-LF complex nanoformulations. Our results indicate that TA binds to LF and forms self-assemblies, which profoundly enhance interaction with LC cells. This study demonstrated that TA is a novel carrier for pharmaceutical drugs such as gemcitabine, carboplatin, and irinotecan.

Original languageEnglish (US)
Article number111
JournalPharmaceutics
Volume10
Issue number3
DOIs
StatePublished - Sep 1 2018

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Tannins
Drug Interactions
Lung Neoplasms
Lung
irinotecan
Pharmaceutical Preparations
gemcitabine
Proteins
Drug Carriers
Carboplatin
Fluorescence Spectrometry
Polyphenols
Biocompatible Materials
Transmission Electron Microscopy
Adsorption
Polyacrylamide Gel Electrophoresis
Cause of Death
Neoplasms
Fluorescence

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

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Tannic acid-lung fluid assemblies promote interaction and delivery of drugs to lung cancer cells. / Hatami, Elham; Nagesh, Prashanth K.B.; Chowdhury, Pallabita; Chauhan, Subhash; Jaggi, Meena; Samarasinghe, Amali; Yallapu, Murali.

In: Pharmaceutics, Vol. 10, No. 3, 111, 01.09.2018.

Research output: Contribution to journalArticle

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