Targeted disruption of one allele of the Y-box protein gene, Chk-YB-1b, in DT40 cells results in major defects in cell cycle

Shivalingappa K. Swamynathan, Balwantkumar R. Varma, Karl Weber, Ramareddy Guntaka

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Y-box or inverted CCAAT box-binding proteins are multifunctional regulators of transcription and translation of several genes. Although YB-1 has been shown to play a key role in cell cycle, to date, there is no direct evidence. We disrupted one allele of Chk-YB-1b in a chicken pre-B lymphocyte cell line, DT40. Compared to wild-type DT40 cells, these heterozygous DT40YB1b+/- cells with one copy of the wild-type Chk-YB-1b allele showed multiple abnormalities, which include slower rate of growth, abnormal cell morphology, increased cell size, and increased genomic DNA content. These phenotypic defects resemble those cells that have a block in G2 and/or mitosis (G2/M). In addition, we have observed that a fraction of these heterozygous DT40YB1b+/- cells undergo apoptosis. In conclusion, we have discovered major defects in the G2/M phase of cell cycle in YB-1 knocked-out heterozygous mutant cells, providing for the first time direct evidence establishing a crucial role for YB-1 in cell proliferation.

Original languageEnglish (US)
Pages (from-to)451-457
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume296
Issue number2
DOIs
StatePublished - Oct 14 2002

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Cell Cycle
Alleles
Cells
Defects
Proteins
B-Lymphoid Precursor Cells
Lymphocytes
Cell proliferation
Cell growth
Transcription
Mitosis
Carrier Proteins
Genes
Apoptosis
Multiple Abnormalities
DNA
Cell Size
Chickens
Cell Proliferation
Cell Line

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Targeted disruption of one allele of the Y-box protein gene, Chk-YB-1b, in DT40 cells results in major defects in cell cycle. / Swamynathan, Shivalingappa K.; Varma, Balwantkumar R.; Weber, Karl; Guntaka, Ramareddy.

In: Biochemical and Biophysical Research Communications, Vol. 296, No. 2, 14.10.2002, p. 451-457.

Research output: Contribution to journalArticle

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AU - Weber, Karl

AU - Guntaka, Ramareddy

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