Taurine activates delayed rectifier KV channels via a metabotropic pathway in retinal neurons

Simon Bulley, Yufei Liu, Harris Ripps, Wen Shen

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Taurine is one of the most abundant amino acids in the retina, throughout the CNS, and in heart and muscle cells. In keeping with its broad tissue distribution, taurine serves as a modulator of numerous basic processes, such as enzyme activity, cell development, myocardial function and cytoprotection. Despite this multitude of functional roles, the precise mechanism underlying taurine's actions has not yet been identified. In this study we report findings that indicate a novel role for taurine in the regulation of voltage-gated delayed rectifier potassium (KV) channels in retinal neurons by means of a metabotropic receptor pathway. The metabotropic taurine response was insensitive to the Cl- channel blockers, picrotoxin and strychnine, but it was inhibited by a specific serotonin 5-HT2A receptor antagonist, MDL11939. Moreover, we found that taurine enhanced KV channels via intracellular protein kinase C-mediated pathways. When 5-HT2A receptors were expressed in human embryonic kidney cells, taurine and AL34662, a non-specific 5-HT2 receptor activator, produced a similar regulation of KIR channels. In sum, this study provides new evidence that taurine activates a serotonin system, apparently via 5-HT2A receptors and related intracellular pathways.

Original languageEnglish (US)
Pages (from-to)123-132
Number of pages10
JournalJournal of Physiology
Volume591
Issue number1
DOIs
StatePublished - Jan 1 2013

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Retinal Neurons
Taurine
Receptor, Serotonin, 5-HT2A
Delayed Rectifier Potassium Channels
Serotonin 5-HT2 Receptor Antagonists
Voltage-Gated Potassium Channels
Strychnine
Picrotoxin
Cytoprotection
Tissue Distribution
Cardiac Myocytes
Protein Kinase C
Retina
Serotonin
Kidney
Amino Acids

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

Taurine activates delayed rectifier KV channels via a metabotropic pathway in retinal neurons. / Bulley, Simon; Liu, Yufei; Ripps, Harris; Shen, Wen.

In: Journal of Physiology, Vol. 591, No. 1, 01.01.2013, p. 123-132.

Research output: Contribution to journalArticle

Bulley, Simon ; Liu, Yufei ; Ripps, Harris ; Shen, Wen. / Taurine activates delayed rectifier KV channels via a metabotropic pathway in retinal neurons. In: Journal of Physiology. 2013 ; Vol. 591, No. 1. pp. 123-132.
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