TEM morphological characterization of a one-step self-etching system applied clinically to human caries-affected dentin and deep sound dentin

Egle Milia, Roberto Pinna, Giorgio Castelli, Antonella Bortone, Salvatore Marceddu, Franklin Garcia-Godoy, Giuseppe Gallina

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Purpose: To examine morphologically the hybrid layer of one-step self-etching adhesive, Clearfil S3 Bond (S3bond), in caries-affected dentin (CAD) and deep sound dentin (DSD) cavities performed clinically. For a comparative group, the two-step self etching adhesive Clearfil Protect Bond (Pbond) was used in a similar clinical situation. Methods: This study was carried out on carious and sound teeth clinically selected for extraction. In carious teeth, CAD was obtained by way of subjective criteria by removing infected tissue to form a cavity bottom. DSD was obtained at a depth of 4 mm in the dentin cavity of sound teeth. S3bond and Pbond were applied in the CAD and DSD cavities as indicated by the manufacturer, followed by a composite restoration. Teeth were extracted about 20 minutes after the bonding procedure, and processed for TEM analysis. Results: Expression of S 3bond in CAD was morphologically highly variable. When affected tubules were occluded by intratubular mineralized deposits, the interface displayed a dense poly-HEMA hydrogel as water sorption by the hydrophilic S 3bond toward the porous affected collagen. Conversely, when tubules appeared empty, voids of various sizes were formed by tubular fluid shift. In DSD, S bond clearly exhibited voids and water channels as signs of the high permeability of the sub-surface. Although porosities were somewhat retained, Pbond expression showed a hermetic character which was independent of the dentin substrate. S3bond bonding to dentin was compromised as shown by signs of water movement within the resin/dentin bond outlining a weak capacity to produce an impermeable hybridiztion.

Original languageEnglish (US)
Pages (from-to)321-326
Number of pages6
JournalAmerican Journal of Dentistry
Volume25
Issue number6
StatePublished - Dec 1 2012

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Dentin
Tooth
Adhesives
Fluid Shifts
Water Movements
Aquaporins
Hydrogel
Porosity
Permeability
Collagen

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Dentistry(all)

Cite this

TEM morphological characterization of a one-step self-etching system applied clinically to human caries-affected dentin and deep sound dentin. / Milia, Egle; Pinna, Roberto; Castelli, Giorgio; Bortone, Antonella; Marceddu, Salvatore; Garcia-Godoy, Franklin; Gallina, Giuseppe.

In: American Journal of Dentistry, Vol. 25, No. 6, 01.12.2012, p. 321-326.

Research output: Contribution to journalArticle

Milia, Egle ; Pinna, Roberto ; Castelli, Giorgio ; Bortone, Antonella ; Marceddu, Salvatore ; Garcia-Godoy, Franklin ; Gallina, Giuseppe. / TEM morphological characterization of a one-step self-etching system applied clinically to human caries-affected dentin and deep sound dentin. In: American Journal of Dentistry. 2012 ; Vol. 25, No. 6. pp. 321-326.
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abstract = "Purpose: To examine morphologically the hybrid layer of one-step self-etching adhesive, Clearfil S3 Bond (S3bond), in caries-affected dentin (CAD) and deep sound dentin (DSD) cavities performed clinically. For a comparative group, the two-step self etching adhesive Clearfil Protect Bond (Pbond) was used in a similar clinical situation. Methods: This study was carried out on carious and sound teeth clinically selected for extraction. In carious teeth, CAD was obtained by way of subjective criteria by removing infected tissue to form a cavity bottom. DSD was obtained at a depth of 4 mm in the dentin cavity of sound teeth. S3bond and Pbond were applied in the CAD and DSD cavities as indicated by the manufacturer, followed by a composite restoration. Teeth were extracted about 20 minutes after the bonding procedure, and processed for TEM analysis. Results: Expression of S 3bond in CAD was morphologically highly variable. When affected tubules were occluded by intratubular mineralized deposits, the interface displayed a dense poly-HEMA hydrogel as water sorption by the hydrophilic S 3bond toward the porous affected collagen. Conversely, when tubules appeared empty, voids of various sizes were formed by tubular fluid shift. In DSD, S bond clearly exhibited voids and water channels as signs of the high permeability of the sub-surface. Although porosities were somewhat retained, Pbond expression showed a hermetic character which was independent of the dentin substrate. S3bond bonding to dentin was compromised as shown by signs of water movement within the resin/dentin bond outlining a weak capacity to produce an impermeable hybridiztion.",
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AU - Garcia-Godoy, Franklin

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