Temporal thresholds for neocortical infarction in rats subjected to reversible focal cerebral ischemia

B. Kaplan, S. Brint, J. Tanabe, Michael Jacewicz, X. J. Wang, W. Pulsinelli

Research output: Contribution to journalArticle

259 Citations (Scopus)

Abstract

We investigated the temporal threshold for focal cerebral infarction in the spontaneously hypertensive rat The right middle cerebral artery and common carotid artery were occluded for 0, 1, 2, 3, 4, or 24 hours, and all the animals were sacrificed 24 hours after the onset of ischemia. Cortical infarct volumes and edema volumes were quantified in serial frozen sections of hematoxylin and eosin-stained tissue using image analysis. Upon occlusion, blood flow in the core of the ischemic zone, measured with laser-Doppler flowmetry, fell to a mean±standard deviation of 21 ± 7% of the preocclusion baseline value (n=26). During the first hour of ischemia, blood flow in the densely ischemic zone rose to 27 ± 8% of baseline (n=25). Release of the middle cerebral artery and common carotid artery occlusions rapidly restored cortical blood flow to 213 ± 83% of baseline (n=21). Focal ischemia of 1 hour’s duration caused little or no infarction, while ischemic intervals of 2 and 3 hours produced successively larger volumes of infarcted cortex. Ischemic intervals of 3-4 hours’ duration followed by approximately 20 hours of recirculation yielded infarct volumes that were not significantly different from those after 24 hours of permanent focal ischemia. The results indicate that 3-4 hours of focal cerebral ischemia in this rat model is sufficient to attain maximal infarction and suggest that recirculation or pharmacological interventions after this time will provide little benefit.

Original languageEnglish (US)
Pages (from-to)1032-1039
Number of pages8
JournalStroke
Volume22
Issue number8
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

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Brain Ischemia
Infarction
Ischemia
Common Carotid Artery
Middle Cerebral Artery
Laser-Doppler Flowmetry
Cerebral Infarction
Frozen Sections
Inbred SHR Rats
Hematoxylin
Eosine Yellowish-(YS)
Edema
Pharmacology

All Science Journal Classification (ASJC) codes

  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine
  • Advanced and Specialized Nursing

Cite this

Temporal thresholds for neocortical infarction in rats subjected to reversible focal cerebral ischemia. / Kaplan, B.; Brint, S.; Tanabe, J.; Jacewicz, Michael; Wang, X. J.; Pulsinelli, W.

In: Stroke, Vol. 22, No. 8, 01.01.1991, p. 1032-1039.

Research output: Contribution to journalArticle

Kaplan, B. ; Brint, S. ; Tanabe, J. ; Jacewicz, Michael ; Wang, X. J. ; Pulsinelli, W. / Temporal thresholds for neocortical infarction in rats subjected to reversible focal cerebral ischemia. In: Stroke. 1991 ; Vol. 22, No. 8. pp. 1032-1039.
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