Testis biopsy image analysis effectively quantifies spermatogenic cell types

Edward Kim, William W. Lin, Jacki Abrams, Larry I. Lipshultz

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Purpose: Evaluation of the testis biopsy specimen in male infertility has been hampered by the qualitative rather than quantitative nature of routine histological techniques and interpretation. Although useful, flow cytometry has demonstrated significant limitations due to its inability to differentiate among haploid cell types, spermatozoa and spermatids. Thus, deoxyribonucleic acid image analysis was performed on testis biopsy specimens to combine the advantages of quantitative ploidy and morphological characteristics to develop a more effective quantitative system. Materials and Methods: Image analysis using a specifically designed filter was performed on Feulgen stained 5 μm. sections of paraffin embedded testicular tissue. The archival testicular tissue had been obtained using standard biopsy techniques from patients with azoospermia and normal spermatogenesis on routine hematoxylin and eosin processed tissue. Results: The overall findings (mean plus or minus standard deviation) in 20 biopsies from 18 men are haploid 54.2 ± 5.6, diploid 31.2 ± 5.2 and tetraploid 15.0 ± 4.6%. Spermatozoa and spermatids contributed 23.2 ± 5.7 and 30.9 ± 7.6% to the total cell content, respectively. A mean of 2,462 ± 254 cells were counted per case. Conclusions: We used image analysis to evaluate testicular biopsies. This technique can quantitatively determine the percentages of various cell types within the seminiferous tubules. Furthermore, it can differentiate between spermatozoa and spermatids based on morphological characteristics. This technique, which combines ploidy and cell morphology characteristics, represents a significant advancement in the interpretation of the testicular biopsy, and has implications for redefining the presently used system of qualitative interpretative assessment.

Original languageEnglish (US)
Pages (from-to)147-150
Number of pages4
JournalJournal of Urology
Volume157
Issue number1
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

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Testis
Biopsy
Spermatids
Spermatozoa
Ploidies
Haploidy
Histological Techniques
Azoospermia
Seminiferous Tubules
Tetraploidy
Male Infertility
Spermatogenesis
Hematoxylin
Eosine Yellowish-(YS)
Diploidy
Paraffin
Flow Cytometry
DNA

All Science Journal Classification (ASJC) codes

  • Urology

Cite this

Testis biopsy image analysis effectively quantifies spermatogenic cell types. / Kim, Edward; Lin, William W.; Abrams, Jacki; Lipshultz, Larry I.

In: Journal of Urology, Vol. 157, No. 1, 01.01.1997, p. 147-150.

Research output: Contribution to journalArticle

Kim, Edward ; Lin, William W. ; Abrams, Jacki ; Lipshultz, Larry I. / Testis biopsy image analysis effectively quantifies spermatogenic cell types. In: Journal of Urology. 1997 ; Vol. 157, No. 1. pp. 147-150.
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abstract = "Purpose: Evaluation of the testis biopsy specimen in male infertility has been hampered by the qualitative rather than quantitative nature of routine histological techniques and interpretation. Although useful, flow cytometry has demonstrated significant limitations due to its inability to differentiate among haploid cell types, spermatozoa and spermatids. Thus, deoxyribonucleic acid image analysis was performed on testis biopsy specimens to combine the advantages of quantitative ploidy and morphological characteristics to develop a more effective quantitative system. Materials and Methods: Image analysis using a specifically designed filter was performed on Feulgen stained 5 μm. sections of paraffin embedded testicular tissue. The archival testicular tissue had been obtained using standard biopsy techniques from patients with azoospermia and normal spermatogenesis on routine hematoxylin and eosin processed tissue. Results: The overall findings (mean plus or minus standard deviation) in 20 biopsies from 18 men are haploid 54.2 ± 5.6, diploid 31.2 ± 5.2 and tetraploid 15.0 ± 4.6{\%}. Spermatozoa and spermatids contributed 23.2 ± 5.7 and 30.9 ± 7.6{\%} to the total cell content, respectively. A mean of 2,462 ± 254 cells were counted per case. Conclusions: We used image analysis to evaluate testicular biopsies. This technique can quantitatively determine the percentages of various cell types within the seminiferous tubules. Furthermore, it can differentiate between spermatozoa and spermatids based on morphological characteristics. This technique, which combines ploidy and cell morphology characteristics, represents a significant advancement in the interpretation of the testicular biopsy, and has implications for redefining the presently used system of qualitative interpretative assessment.",
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