Tetraspanin CD151 maintains vascular stability by balancing the forces of cell adhesion and cytoskeletal tension

Feng Zhang, Jarett E. Michaelson, Simon Moshiach, Norman Sachs, Wenyuan Zhao, Yao Sun, Arnoud Sonnenberg, Jill M. Lahti, Hayden Huang, Xin A. Zhang

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Tetraspanin CD151 is highly expressed in endothelial cells and regulates pathologic angiogenesis. However, the mechanism by which CD151 promotes vascular morphogenesis and whether CD151 engages other vascular functions are unclear. Here we report that CD151 is required for maintaining endothelial capillary-like structures formed in vitro and the integrity of endothelial cell-cell and cell-matrix contacts in vivo. In addition, vascular permeability is markedly enhanced in the absence of CD151. As a global regulator of endothelial cell-cell and cell-matrix adhesions, CD151 is needed for the optimal functions of various cell adhesion proteins. The loss of CD151 elevates actin cytoskeletal traction by up-regulating RhoA signaling and diminishes actin cortical meshwork by down-regulating Rac1 activity. The inhibition of RhoA or activation of cAMP signaling stabilizes CD151-silenced or -null endothelial structure in vascular morphogenesis. Together, our data demonstrate that CD151 maintains vascular stability by promoting endothelial cell adhesions, especially cell-cell adhesion, and confining cytoskeletal tension.

Original languageEnglish (US)
Pages (from-to)4274-4284
Number of pages11
JournalBlood
Volume118
Issue number15
DOIs
StatePublished - Oct 13 2011

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Cell adhesion
Endothelial cells
Cell Adhesion
Blood Vessels
Endothelial Cells
Morphogenesis
Actins
Pathologic Neovascularization
Cell-Matrix Junctions
Capillary Permeability
Traction
Adhesion
Chemical activation
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Zhang, F., Michaelson, J. E., Moshiach, S., Sachs, N., Zhao, W., Sun, Y., ... Zhang, X. A. (2011). Tetraspanin CD151 maintains vascular stability by balancing the forces of cell adhesion and cytoskeletal tension. Blood, 118(15), 4274-4284. https://doi.org/10.1182/blood-2011-03-339531

Tetraspanin CD151 maintains vascular stability by balancing the forces of cell adhesion and cytoskeletal tension. / Zhang, Feng; Michaelson, Jarett E.; Moshiach, Simon; Sachs, Norman; Zhao, Wenyuan; Sun, Yao; Sonnenberg, Arnoud; Lahti, Jill M.; Huang, Hayden; Zhang, Xin A.

In: Blood, Vol. 118, No. 15, 13.10.2011, p. 4274-4284.

Research output: Contribution to journalArticle

Zhang, F, Michaelson, JE, Moshiach, S, Sachs, N, Zhao, W, Sun, Y, Sonnenberg, A, Lahti, JM, Huang, H & Zhang, XA 2011, 'Tetraspanin CD151 maintains vascular stability by balancing the forces of cell adhesion and cytoskeletal tension', Blood, vol. 118, no. 15, pp. 4274-4284. https://doi.org/10.1182/blood-2011-03-339531
Zhang, Feng ; Michaelson, Jarett E. ; Moshiach, Simon ; Sachs, Norman ; Zhao, Wenyuan ; Sun, Yao ; Sonnenberg, Arnoud ; Lahti, Jill M. ; Huang, Hayden ; Zhang, Xin A. / Tetraspanin CD151 maintains vascular stability by balancing the forces of cell adhesion and cytoskeletal tension. In: Blood. 2011 ; Vol. 118, No. 15. pp. 4274-4284.
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