Tetraspanin CD151 promotes cell migration by regulating integrin trafficking

Li Liu, Bo He, Wei M. Liu, Dongming Zhou, John Cox, Xin A. Zhang

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Regulation of cell migration is an important feature of tetraspanin CD151. Although it is well established that CD151 physically associates with integrins, the mechanism by which CD151 regulates integrin-dependent cell migration is basically unknown. Given the fact that CD151 is localized in both the plasma membrane and intracellular vesicles, we found that CD151 and its associated α3β1, α5β1, and α6β1 integrins undergo endocytosis and accumulate in the same intracellular vesicular compartments. CD151 contains a YRSL sequence, a YXXφ type of endocytosis/sorting motif, in its C-terminal cytoplasmic domain. Mutation of this motif markedly attenuated CD151 internalization. The loss of CD151 trafficking completely abrogated CD151-promoted cell migration on extracellular matrices such as laminin and diminished the internalization of its associated integrins, indicating a critical role for integrin trafficking in regulating cell motility. In conclusion, the YXXφ motif-mediated internalization of CD151 promotes integrin-dependent cell migration by modulating the endocytosis and/or vesicular trafficking of its associated integrins.

Original languageEnglish (US)
Pages (from-to)31631-31642
Number of pages12
JournalJournal of Biological Chemistry
Volume282
Issue number43
DOIs
StatePublished - Oct 26 2007

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Integrins
Cell Movement
Endocytosis
Laminin
Cell membranes
Sorting
Extracellular Matrix
Cells
Cell Membrane
Mutation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Tetraspanin CD151 promotes cell migration by regulating integrin trafficking. / Liu, Li; He, Bo; Liu, Wei M.; Zhou, Dongming; Cox, John; Zhang, Xin A.

In: Journal of Biological Chemistry, Vol. 282, No. 43, 26.10.2007, p. 31631-31642.

Research output: Contribution to journalArticle

Liu, Li ; He, Bo ; Liu, Wei M. ; Zhou, Dongming ; Cox, John ; Zhang, Xin A. / Tetraspanin CD151 promotes cell migration by regulating integrin trafficking. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 43. pp. 31631-31642.
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