TFIIB-facilitated recruitment of preinitiation complexes by a TAF-independent mechanism

Roderick Hori, Shuping Xu, Xianyuan Hu, Sung Pyo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Gene activators contain activation domains that are thought to recruit limiting components of the transcription machinery to a core promoter. VP16, a viral gene activator, has served as a model for studying the mechanistic aspects of transcriptional activation from yeast to human. The VP16 activation domain can be divided into two modules - an N-terminal subdomain (VPN) and a C-terminal subdomain (VPC). This study demonstrates that VPC stimulates core promoters that are either independent or dependent on TAFs (TATA-box Binding Protein-Associated Factors). In contrast, VPN only activates the TAF-independent core promoter and this activity increases in a synergistic fashion when VPN is dimerized (VPN2). Compared to one copy of VPN (VPN1), VPN2 also displays a highly cooperative increase in binding hTFIIB. The increased TFIIB binding correlates with VPN2's increased ability to recruit a complex containing TFIID, TFIIA and TFIIB. However, VPN1 and VPN2 do not increase the assembly of a complex containing only TFIID and TFIIA. The VPN subdomain also facilitates assembly of a complex containing TBP: TFIIA:TFIIB, which lacks TAFs, and provides a mechanism that could function at TAF-independent promoters. Taken together, these results suggest the interaction between VPN and TFIIB potentially initiate a network of contacts allowing the activator to indirectly tether TFIID or TBP to DNA.

Original languageEnglish (US)
Pages (from-to)3856-3863
Number of pages8
JournalNucleic acids research
Volume32
Issue number13
DOIs
StatePublished - Sep 21 2004

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TATA-Binding Protein Associated Factors
Transcription Factor TFIIB
TATA-Box Binding Protein
Transcription Factor TFIIA
Transcription Factor TFIID
Viral Genes
Transcriptional Activation
Yeasts
DNA
Genes

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

TFIIB-facilitated recruitment of preinitiation complexes by a TAF-independent mechanism. / Hori, Roderick; Xu, Shuping; Hu, Xianyuan; Pyo, Sung.

In: Nucleic acids research, Vol. 32, No. 13, 21.09.2004, p. 3856-3863.

Research output: Contribution to journalArticle

Hori, Roderick ; Xu, Shuping ; Hu, Xianyuan ; Pyo, Sung. / TFIIB-facilitated recruitment of preinitiation complexes by a TAF-independent mechanism. In: Nucleic acids research. 2004 ; Vol. 32, No. 13. pp. 3856-3863.
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