The β4 subunit of the integrin family is displayed on a restricted subset of endothelium in mice

Stephen Kennel, V. Godfrey, L. Y. Ch'ang, T. K. Lankford, L. J. Foote, A. Makkinje

Research output: Contribution to journalArticle

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Abstract

More than 15 subunits of the integrin family of cell surface adhesion molecules have been identified. The α6β4 integrin has recently been identified as a component of hemidesmosomes of stratified squamous epithelium. The monoclonal antibody (mAb) 346-11A binds to the β4 subunit in mice. Sequence analysis of a cDNA clone coding for this epitope localizes reaction of the mAb to a portion about half way through the extracellular domain at the beginning of the cysteine-rich region. Sites of β4 expression in mice were detected by autoradiographic analysis of tissues collected from mice 24 or 48h after intravenous injection of 125I-labeled mAb 346-11A. This non-quantitative technique emphasizes detection of antigen exposed in the vascular space. These data show that in addition to the epithelia of several organs, the endothelia of intermediate vessels throughout the body are sites of β4 expression. In particular, endothelium of larger vessels but not capillaries in lung, vessels in thymus, spleen and Peyer's patches, and portal vessels but not the central veins of the liver, are positive. The expression of the β4 integrin in blood vessels may indicate a specialized function for β4 at these sites that is distinct from its role in hemidesmosome-mediated attachment.

Original languageEnglish (US)
Pages (from-to)145-150
Number of pages6
JournalJournal of Cell Science
Volume101
Issue number1
StatePublished - 1992
Externally publishedYes

Fingerprint

Integrins
Hemidesmosomes
Endothelium
Monoclonal Antibodies
Blood Vessels
Epithelium
Peyer's Patches
Cell Adhesion Molecules
Intravenous Injections
Thymus Gland
Cysteine
Sequence Analysis
Epitopes
Veins
Spleen
Complementary DNA
Clone Cells
Antigens
Lung
Liver

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Kennel, S., Godfrey, V., Ch'ang, L. Y., Lankford, T. K., Foote, L. J., & Makkinje, A. (1992). The β4 subunit of the integrin family is displayed on a restricted subset of endothelium in mice. Journal of Cell Science, 101(1), 145-150.

The β4 subunit of the integrin family is displayed on a restricted subset of endothelium in mice. / Kennel, Stephen; Godfrey, V.; Ch'ang, L. Y.; Lankford, T. K.; Foote, L. J.; Makkinje, A.

In: Journal of Cell Science, Vol. 101, No. 1, 1992, p. 145-150.

Research output: Contribution to journalArticle

Kennel, S, Godfrey, V, Ch'ang, LY, Lankford, TK, Foote, LJ & Makkinje, A 1992, 'The β4 subunit of the integrin family is displayed on a restricted subset of endothelium in mice', Journal of Cell Science, vol. 101, no. 1, pp. 145-150.
Kennel, Stephen ; Godfrey, V. ; Ch'ang, L. Y. ; Lankford, T. K. ; Foote, L. J. ; Makkinje, A. / The β4 subunit of the integrin family is displayed on a restricted subset of endothelium in mice. In: Journal of Cell Science. 1992 ; Vol. 101, No. 1. pp. 145-150.
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