The absence of the VPAC2 receptor does not protect mice from Aspergillus induced allergic asthma

Amali Samarasinghe, S. A. Hoselton, J. M. Schuh

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Allergic asthma is a TH2-mediated disease marked by airway inflammation, increased mucus production, and elevated serum IgE in response to allergen provocation. Among its ascribed functions, the neuropeptide vasoactive intestinal peptide (VIP) is believed to promote a TH2 phenotype when signaling through its VPAC2 receptor. In this study, we assessed the requirement for the VIP/VPAC2 axis in initiating the allergic pulmonary phenotype in a murine model of fungal allergic asthma. C57BL/6 wild-type (WT) and VPAC2 knock-out (KO) mice were sensitized with Aspergillus fumigatus antigen and challenged with an aerosol of live conidia to induce allergic airways disease. WT and KO mice exhibited similar peribronchovascular inflammation, increased number of goblet cells, and elevated serum IgE. However, the absence of VPAC2 receptor resulted in a marked enhancement of MUC5AC mRNA with an associated increase in goblet cells and a reduction in eosinophils in the airway lumen at day 3 when VIP mRNA was undetectable in the KO lung. Sustained elevation of serum IgE was noted in KO mice at day 14, while the level in WT mice declined at this time point. These data suggest that the absence of VPAC2 does not protect mice from developing the signs and symptoms of allergic asthma.

Original languageEnglish (US)
Pages (from-to)1068-1075
Number of pages8
JournalPeptides
Volume31
Issue number6
DOIs
StatePublished - Jun 1 2010

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Receptors, Vasoactive Intestinal Peptide, Type II
Aspergillus
Vasoactive Intestinal Peptide
Knockout Mice
Immunoglobulin E
Goblet Cells
Asthma
Serum
Inflammation
Phenotype
Lung
Messenger RNA
Aspergillus fumigatus
Fungal Spores
Mucus
Neuropeptides
Aerosols
Eosinophils
Allergens
Signs and Symptoms

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

Cite this

The absence of the VPAC2 receptor does not protect mice from Aspergillus induced allergic asthma. / Samarasinghe, Amali; Hoselton, S. A.; Schuh, J. M.

In: Peptides, Vol. 31, No. 6, 01.06.2010, p. 1068-1075.

Research output: Contribution to journalArticle

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