The absence of VPAC2 leads to aberrant antibody production in Aspergillus fumigatus sensitized and challenged mice

Amali Samarasinghe, Scott A. Hoselton, Jane M. Schuh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Vasoactive intestinal peptide (VIP) facilitates a "pro-allergy" phenotype when signaling through its G protein-coupled receptor, VPAC 2. We have shown that VPAC2 knock-out (KO) mice developed an allergic phenotype marked by eosinophilia and elevated serum IgE. Therefore, we hypothesized that the humoral response to allergen challenge in these mice was TH2 dominant similar to wild-type (WT) C57BL/6 mice. Antibody responses in WT and KO mice were measured after Aspergillus fumigatus conidia inhalation. In contrast to previous reports, basal levels of serum IgG 2a and IgA were significantly higher in naïve VPAC2 KO animals. Antibody availability in the serum as well as the bronchoalveolar lavage fluid after fungal challenge was dominated by the pro-inflammatory isotype IgG2a and the mucosal isotype, IgA. IgA localizing cells dominated in the peribronchovascular areas of allergic KO mice while IgE immune complexes were found in WT allergic lungs. This research shows for the first time that VPAC2 has a significant effect on antibody regulation, in the context of allergy.

Original languageEnglish (US)
Pages (from-to)131-137
Number of pages7
JournalPeptides
Volume32
Issue number1
DOIs
StatePublished - Jan 1 2011

Fingerprint

Aspergillus fumigatus
Aspergillus
Knockout Mice
Immunoglobulin A
Allergies
Antibody Formation
Immunoglobulin E
Antibodies
CCR10 Receptors
Hypersensitivity
Serum
Phenotype
Fungal Spores
Vasoactive Intestinal Peptide
Bronchoalveolar Lavage Fluid
Eosinophilia
Antigen-Antibody Complex
Inbred C57BL Mouse
Allergens
Inhalation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

The absence of VPAC2 leads to aberrant antibody production in Aspergillus fumigatus sensitized and challenged mice. / Samarasinghe, Amali; Hoselton, Scott A.; Schuh, Jane M.

In: Peptides, Vol. 32, No. 1, 01.01.2011, p. 131-137.

Research output: Contribution to journalArticle

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