The allantoin and uracil permease gene sequences of Saccharomyces cerevisiae are nearly identical

Hyang Sook Yoo, Thomas S. Cunningham, Terrance Cooper

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We have determined the structure of the allantoin permease (DAL4) gene of Saccharomyces cerevisiae. The gene patatively encodes a hydrophobic protein with a M r of 71 755. It possesses the alternating hydrophobic–hydrophilic regions similar to those found in many other integral membrane proteins. The most striking feature of the allantoin permease component encoded by DAL4 is its striking similarity to the uracil permease component encoded by FUR4 Although data available indicate that these proteins do not share any overlap of function, their predicted protein sequences are 68% identical, 81% similar, and their DNA sequences are 70% identical. The upstream regulatory region of DAL4 contains all lof the characterized cis‐acting elements previously reported for inducible allantoin pathway genes: six sequences homologous to UAS NTR , the element responsible for nitrogen catabolite repression‐sensitive activation of allantoin pathway gene expression, and two sequences homologous to the cis‐acting element responsible for inducere‐responsiveness of the allantoin pathway genes, UIS. The finding of these homologous sequences predicted to exist on the basis of DAL4's expression characteristics, supports and strengthens the suggestion that these elements mediate the functions we have we have previously ascribed to them.

Original languageEnglish (US)
Pages (from-to)997-1006
Number of pages10
JournalYeast
Volume8
Issue number12
DOIs
StatePublished - Jan 1 1992

Fingerprint

Allantoin
Membrane Transport Proteins
Uracil
Yeast
Saccharomyces cerevisiae
Genes
Sequence Homology
Proteins
Nucleic Acid Regulatory Sequences
DNA sequences
Gene expression
Chemical elements
Membrane Proteins
Nitrogen
Chemical activation
Membranes
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

The allantoin and uracil permease gene sequences of Saccharomyces cerevisiae are nearly identical. / Yoo, Hyang Sook; Cunningham, Thomas S.; Cooper, Terrance.

In: Yeast, Vol. 8, No. 12, 01.01.1992, p. 997-1006.

Research output: Contribution to journalArticle

Yoo, Hyang Sook ; Cunningham, Thomas S. ; Cooper, Terrance. / The allantoin and uracil permease gene sequences of Saccharomyces cerevisiae are nearly identical. In: Yeast. 1992 ; Vol. 8, No. 12. pp. 997-1006.
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