The allantoinase (DAL1) gene of Saccharomyces cerevisiae

Richard G. Buckholz, Terrance Cooper

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The allantoinase (DAL1) gene from Saccharomyces cerevisiae has been cloned, sequenced, and found to encode a 472 amino acid protein with a Mr of 52 028. DAL1 is expressed in an inducer‐independent manner in strain M970 (∑1278b genetic background) and modestly responds to mutation of the da180 locus. Expression was also sensitive to nitrogen catabolite repression (NCR). Correlated with these expression characteristics, the upstream region of DAL1 contained five copies of a sequence that is homolgous to the DAL UASNTR element previously shown to be required for transcriptional activation and NCR sensitivity of the DAL5 and DAL7 genes. Missing from the DAL1 5′ flanking region were any sequences with significant homology to the DAL7 UIS element required for response to inducer. These observations further support the roles of UASNTR and DAL7 UIS in the regulation of allantoin pathway gene expression.

Original languageEnglish (US)
Pages (from-to)913-923
Number of pages11
JournalYeast
Volume7
Issue number9
DOIs
StatePublished - Jan 1 1991

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Catabolite Repression
Yeast
Allantoin
Saccharomyces cerevisiae
Nitrogen
Genes
5' Flanking Region
Response Elements
Gene expression
Transcriptional Activation
Amino acids
Chemical activation
Proteins
Gene Expression
Amino Acids
Mutation
allantoinase
Genetic Background

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

The allantoinase (DAL1) gene of Saccharomyces cerevisiae. / Buckholz, Richard G.; Cooper, Terrance.

In: Yeast, Vol. 7, No. 9, 01.01.1991, p. 913-923.

Research output: Contribution to journalArticle

Buckholz, Richard G. ; Cooper, Terrance. / The allantoinase (DAL1) gene of Saccharomyces cerevisiae. In: Yeast. 1991 ; Vol. 7, No. 9. pp. 913-923.
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