The Association Between Myocardial Fibrosis and Depressed Capillary Density in Rat Model of Left Ventricular Hypertrophy

Ying Xiao, Yinjie Liu, Jiaming Liu, Yujian Kang

Research output: Contribution to journalArticle

Abstract

Myocardial fibrogenesis is initiated once the coordination between oxygen supply and demand is disrupted in pressure overload-induced cardiac hypertrophy. Clinical observations showed that myocardial fibrosis did not evenly occur in the hypertrophic myocardium. The present study was undertaken to specifically address differential vulnerabilities to fibrogenesis of different regions in the myocardium subjected to pressure overload-induced hypertrophy. SD rats were divided into two groups, sham-operated control and ascending artery constriction-induced cardiac hypotrophy. Thirty-four weeks after surgery, rats were sacrificed and hearts were harvested. Myocardial tissues were processed and sequentially sectioned for detection of collagen deposition, myocyte hypertrophy and vascular density analysis. Redundant collagen stained with Sirius red and anti-collagen I antibody was found in the extracellular matrix, but high volume of collagen fraction was largely localized more in posterior and lateral walls than in anterior wall and interventricular septum, which is in accordance with the accumulation of fibroblasts. In association with the differential regional collagen accumulation, the cardiomyocytes were more hypertrophic in the posterior and lateral wall than the other left ventricle. However, the capillary density in the lateral and posterior walls was significantly decreased. The results indicated that the posterior and lateral walls were more vulnerable to fibrogenesis post-pressure overload-induced cardiac hypertrophy, which was associated with the depressed angiogenesis in these two regions.

Original languageEnglish (US)
Pages (from-to)304-311
Number of pages8
JournalCardiovascular Toxicology
Volume18
Issue number4
DOIs
StatePublished - Aug 1 2018
Externally publishedYes

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Left Ventricular Hypertrophy
Rats
Fibrosis
Collagen
Cardiomegaly
Pressure
Hypertrophy
Myocardium
Fibroblasts
Cardiac Myocytes
Constriction
Muscle Cells
Surgery
Heart Ventricles
Extracellular Matrix
Blood Vessels
Arteries
Tissue
Oxygen
Antibodies

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Toxicology
  • Cardiology and Cardiovascular Medicine

Cite this

The Association Between Myocardial Fibrosis and Depressed Capillary Density in Rat Model of Left Ventricular Hypertrophy. / Xiao, Ying; Liu, Yinjie; Liu, Jiaming; Kang, Yujian.

In: Cardiovascular Toxicology, Vol. 18, No. 4, 01.08.2018, p. 304-311.

Research output: Contribution to journalArticle

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