The Bla2 β-lactamase from the live-vaccine strain of Francisella tularensis encodes a functional protein that is only active against penicillin-class β-lactam antibiotics

Xiaowen R. Bina, Chunmei Wang, Mark Miller, James E. Bina

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Francisella tularensis ssp. tularensis is a category A select agent and the causal organism for the zoonotic disease tularemia. The vast majority of F. tularensis isolates are β-lactamase-positive. β-lactamase production is widely believed to be responsible for the inefficacy of β-lactams in the treatment of tularemia. In this study, we report the cloning and characterization of the two chromosomally encoded F. tularensis ssp. holarctica live-vaccine strain (LVS) β-lactamases. The two LVS β-lactamases were homologous to F. tularensis Schu S4 open reading frames FTT0681c and FTT0611c and have been named bla1 LVS and bla2 LVS , respectively. Recombinant expression in Escherichia coli suggested that bla1 LVS did not encode a functional β-lactamase, whereas bla2 LVS encoded a functional β-lactamase that hydrolyzed penicillins but was inactive against third-generation cephalosporins, including cefprozil. As both LVS and Schu S4 were susceptible to cefprozil, we developed three new shuttle vectors based on selection for the production of the Blashv-2 extended-spectrum β-lactamase with cefprozil. The resulting shuttle vectors were suitable for recombinant gene expression and complementation studies in LVS and Schu S4.

Original languageEnglish (US)
Pages (from-to)219-228
Number of pages10
JournalArchives of Microbiology
Volume186
Issue number3
DOIs
StatePublished - Sep 1 2006

Fingerprint

Francisella tularensis
Lactams
Penicillins
Vaccines
Anti-Bacterial Agents
Proteins
Tularemia
Genetic Vectors
Cloning
Zoonoses
Cephalosporins
Gene expression
Escherichia coli
Open Reading Frames
Organism Cloning
Gene Expression

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

The Bla2 β-lactamase from the live-vaccine strain of Francisella tularensis encodes a functional protein that is only active against penicillin-class β-lactam antibiotics. / Bina, Xiaowen R.; Wang, Chunmei; Miller, Mark; Bina, James E.

In: Archives of Microbiology, Vol. 186, No. 3, 01.09.2006, p. 219-228.

Research output: Contribution to journalArticle

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