The Carboxyl-Terminus Directs TAFI48 to the Nucleus and Nucleolus and Associates with Multiple Nuclear Import Receptors

Joseph L. Dynes, Shuping Xu, Sarah Bothner, Jill M. Lahti, Roderick Hori

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The protein complex Selectivity Factor 1, composed of TBP, TAF I48, TAFI63 and TAFI110, is required for rRNA transcription by RNA polymerase I in the nucleolus. The steps involved in targeting Selectivity Factor 1 will be dependent on the transport pathways that are used and the localization signals that direct this trafficking. In order to investigate these issues, we characterized human TAFI48, a subunit of Selectivity Factor 1. By domain analysis of TAFI48, the carboxyl-terminal 51 residues were found to be required for the localization of TAFI48, as well as sufficient to direct Green Fluorescent Protein to the nucleus and nucleolus. The carboxyl-terminus of TAFI48 also has the ability to associate with multiple members of the β-karyopherin family of nuclear import receptors, including importin β (karyopherin β1), transportin (karyopherin β2) and RanBP5 (karyopherin β2), in a Ran-dependent manner. This property of interacting with multiple β-karyopherins has been previously reported for the nuclear localization signals of some ribosomal proteins that are likewise directed to the nucleolus. This study identifies the first nuclear import sequence identified within the TBP-Associated Factor subunits of Selectivity Factor 1.

Original languageEnglish (US)
Pages (from-to)429-438
Number of pages10
JournalJournal of Biochemistry
Volume135
Issue number3
DOIs
StatePublished - Mar 1 2004

Fingerprint

Karyopherins
Cell Nucleus Active Transport
Cytoplasmic and Nuclear Receptors
TATA-Binding Protein Associated Factors
RNA Polymerase I
Nuclear Localization Signals
Aptitude
Ribosomal Proteins
Transcription
Green Fluorescent Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

The Carboxyl-Terminus Directs TAFI48 to the Nucleus and Nucleolus and Associates with Multiple Nuclear Import Receptors. / Dynes, Joseph L.; Xu, Shuping; Bothner, Sarah; Lahti, Jill M.; Hori, Roderick.

In: Journal of Biochemistry, Vol. 135, No. 3, 01.03.2004, p. 429-438.

Research output: Contribution to journalArticle

Dynes, Joseph L. ; Xu, Shuping ; Bothner, Sarah ; Lahti, Jill M. ; Hori, Roderick. / The Carboxyl-Terminus Directs TAFI48 to the Nucleus and Nucleolus and Associates with Multiple Nuclear Import Receptors. In: Journal of Biochemistry. 2004 ; Vol. 135, No. 3. pp. 429-438.
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