THE chemotactic attraction of human fibroblasts to a lymphocyte-derived factor

Research output: Contribution to journalArticle

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Abstract

A quantitative assay that measures fibroblast chemotaxis in vitro is described. Application of this technique has revealed that peripheral blood lymphocytes stimulated by antigen or mitogen in vitro produce a factor that is chemotactic for human dermal fibroblasts. This lymphocyte-derived chemotactic factor for fibroblasts (LDCF-F) is different from the lymphokine that is chemotactic for monocytes or macrophages. Macrophages are required for the generation of LDCF-F by T lymphocytes stimulated by phytohemagglutinin. The fibroblast chemotactic factor is heat stable (56°C for 30 min), trypsin sensitive, and neuraminidase resistant. LDCF-F could function to attact connective tissue fibroblasts to sites at which cell-mediated immune reactions are occurring in vivo.

Original languageEnglish (US)
Pages (from-to)1188-1203
Number of pages16
JournalJournal of Experimental Medicine
Volume144
Issue number5
DOIs
StatePublished - Nov 2 1976

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Fibroblasts
Lymphocytes
Chemotactic Factors
Macrophages
Lymphokines
Neuraminidase
Phytohemagglutinins
Chemotaxis
Mitogens
Connective Tissue
Trypsin
Monocytes
Hot Temperature
T-Lymphocytes
Antigens
Skin
lymphotactin
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

THE chemotactic attraction of human fibroblasts to a lymphocyte-derived factor. / Postlethwaite, Arnold; Snyderman, Ralph; Kang, Andrew.

In: Journal of Experimental Medicine, Vol. 144, No. 5, 02.11.1976, p. 1188-1203.

Research output: Contribution to journalArticle

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