The Comparison of the Effect of Endodontic Irrigation on Cell Adherence to Root Canal Dentin

Karla C. Ring, Peter E. Murray, Kenneth N. Namerow, Sergio Kuttler, Franklin Garcia-Godoy

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The purpose of this study was to compare the effect of 10 different endodontic irrigation and chelating treatments on dental pulp stem cell (DPSC) attachment to root canal surfaces. Thirty-eight extracted human nondiseased single-canal teeth were cleaned and shaped using ProTaper and ProFile rotary instrumentation (Tulsa Dentsply, Tulsa, OK). The irrigation treatments investigated were 6% sodium hypochlorite, 2% chlorhexidine gluconate, Aquatine Endodontic Cleanser, and Morinda citrifolia juice. The irrigation treatments were used in conjunction with EDTA or MTAD. The instrumented teeth were immediately placed in cell culture with confluent DPSCs for 1 week. The number of attached DPSCs appeared to be correlated with the cytotoxicity of the root canal irrigating solution (analysis of variance, p < 0.0001). The presence or absence of the smear layer had little influence on DPSC activity (chi-square, p > 0.05). The results suggest that biocompatible irrigants are needed to promote DPSC attachment to root canal dentin, which is essential to accomplish some regenerative endodontic therapies.

Original languageEnglish (US)
Pages (from-to)1474-1479
Number of pages6
JournalJournal of Endodontics
Volume34
Issue number12
DOIs
StatePublished - Dec 1 2008
Externally publishedYes

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Dental Pulp Cavity
Endodontics
Dentin
Dental Pulp
Tooth
Stem Cells
Morinda
Sodium Hypochlorite
Edetic Acid
Analysis of Variance
Cell Culture Techniques
Therapeutics

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

The Comparison of the Effect of Endodontic Irrigation on Cell Adherence to Root Canal Dentin. / Ring, Karla C.; Murray, Peter E.; Namerow, Kenneth N.; Kuttler, Sergio; Garcia-Godoy, Franklin.

In: Journal of Endodontics, Vol. 34, No. 12, 01.12.2008, p. 1474-1479.

Research output: Contribution to journalArticle

Ring, Karla C. ; Murray, Peter E. ; Namerow, Kenneth N. ; Kuttler, Sergio ; Garcia-Godoy, Franklin. / The Comparison of the Effect of Endodontic Irrigation on Cell Adherence to Root Canal Dentin. In: Journal of Endodontics. 2008 ; Vol. 34, No. 12. pp. 1474-1479.
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