The DAL81 gene product is required for induced expression of two differently regulated nitrogen catabolic genes in Saccharomyces cerevisiae

P. A. Bricmont, J. R. Daugherty, Terrance Cooper

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

We demonstrate that the DAL81 gene, previously thought to be specifically required for induced expression of the allantoin pathway genes in Saccharomyces cerevisiae, functions in a more global manner. The data presented show it to be required for utilization of 4-aminobutyrate as a nitrogen source and for 4-aminobutyrate-induced increases in the steady-state levels of UGA1 mRNA. The DAL81 gene encodes a 970-amino-acid protein containing sequences homologous to the Zn(II)2Cys6 motif and two stretches of polyglutamine residues. Deletion of sequences homologous to the Zn(II)2Cys6 motif did not result in a detectable loss of function. On the other hand, loss of one of the polyglutamine stretches, but not the other, resulted in a 50% loss of DAL81 function.

Original languageEnglish (US)
Pages (from-to)1161-1166
Number of pages6
JournalMolecular and Cellular Biology
Volume11
Issue number2
DOIs
StatePublished - Jan 1 1991

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Aminobutyrates
Saccharomyces cerevisiae
Nitrogen
Sequence Homology
Allantoin
Genes
Sequence Deletion
Amino Acids
Messenger RNA
Proteins
polyglutamine

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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The DAL81 gene product is required for induced expression of two differently regulated nitrogen catabolic genes in Saccharomyces cerevisiae. / Bricmont, P. A.; Daugherty, J. R.; Cooper, Terrance.

In: Molecular and Cellular Biology, Vol. 11, No. 2, 01.01.1991, p. 1161-1166.

Research output: Contribution to journalArticle

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