The DAL82 protein of Saccharomyces cerevisiae binds to the DAL upstream induction sequence (UIS)

Rosemary A. Dorrington, Terrance Cooper

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Expression of the DAL2, DAL4, DAL7, DUR1.2, and DUR3 genes n S.cerevisiae is induced by allophanate, the last intermediate in the allantoin catabolic pathway. Analysis of the DAL7 promoter identified a dodecanucleotide, the DAL7 UIS, which was required for inducer-responsiveness. Operation of the DAL7 UIS required functional DAL81 and DAL82 gene products. Since the DAL81 product was not an allantoin pathwayspecific regulatory factor, the DAL82 product was considered as the more likely candidate to be the DAL UIS binding protein. Using an E.coli expression system, we showed that DAL82 protein specifically bound to wild type but not mutant DAL UIS sequences. DNA fragments containing DAL UIS elements derived from various DAL gene promoters bound DAL82 protein with different affinities which correlate with the degree of inducer-responsiveness the genes displayed.

Original languageEnglish (US)
Pages (from-to)3777-3784
Number of pages8
JournalNucleic acids research
Volume21
Issue number16
DOIs
StatePublished - Aug 11 1993

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Saccharomyces cerevisiae Proteins
Allantoin
Genes
Saccharomyces cerevisiae
Carrier Proteins
Proteins
Escherichia coli
DNA

All Science Journal Classification (ASJC) codes

  • Genetics

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The DAL82 protein of Saccharomyces cerevisiae binds to the DAL upstream induction sequence (UIS). / Dorrington, Rosemary A.; Cooper, Terrance.

In: Nucleic acids research, Vol. 21, No. 16, 11.08.1993, p. 3777-3784.

Research output: Contribution to journalArticle

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